Whole-transcriptome sequencing and bioinformatics analysis of calcified outer-layer tissue from cystic echinococcosis
https://doi.org/10.47665/tb.41.1.003
- Author:
Wang, S.B.
1
;
Sun, H.H.
2
;
Ma, Y.B.
2
;
Dai, Y.
1
;
Ren, Q.
2
;
Liu, Y.Q.
2
;
Shi, C.H.
1
;
Tao, J.
3
;
Li, J.
1
Author Information
1. Orthopaedic Center of the First Affiliated Hospital of Shihezi University, Shihezi 832000, Xinjiang, China
2. Laboratory of Translational Medicine, School of Medicine, Shihezi University, Shihezi 832000, Xinjiang, China
3. Department of joint surgery, Shihezi peoples Hospital, Shihezi 832000, Xinjiang, China
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
calcification;
cyst wall;
RNA;
apoptosis.
- From:Tropical Biomedicine
2024;41(No.1):20-28
- CountryMalaysia
- Language:English
-
Abstract:
Echinococcosis is a common zoonotic disease in livestock; the type with the highest incidence is cystic
echinococcosis (CE). In clinical management, patients with CE of the liver in which the cyst wall is calcified
have been found to have better prognoses than those without calcification. In this study, we collected
calcified and uncalcified cyst wall tissue from patients with hepatic CE and observed significant changes
in the expression of 2336 messenger ribonucleic acids (mRNAs), 178 long noncoding RNAs (lncRNAs),
210 microRNAs (miRNAs), and 33 circular RNAs (circRNAs) using high-throughput sequencing (HTS).
Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of
differentially expressed RNAs (DERNAs: DEmRNAs, DElncRNAs, DEmiRNAs, and DEcircRNAs) were
performed to explore these RNAs’ potential biological functions and signaling pathways. Ultimately,
the results of hematoxylin and eosin (H&E) and terminal deoxynucleotidyl transferase deoxyuridine
triphosphate (dUTP) nick end labeling (TUNEL) staining confirmed the correlation between calcification
and apoptosis of the cyst wall. In summary, this study was an initial exploration of the molecular-biological
mechanism underlying spontaneous calcification of the hydatid cyst wall, and it provides a theoretical
basis for exploring new targets for drug treatment in CE.
- Full text:20240710121810059268.2024my1449.pdf
