Effects of NADPH oxidase inhibitor of the Etlingera elatior (E. elatior) fruits extracts in animal sepsis models
https://doi.org/10.47665/tb.41.1.002
- Author:
Nurhayatun, E.
1
;
Purwanto, B.
2
;
Ulfia, M.
2
Author Information
1. Division of Tropical and Infection Disease, Department of Internal Medicine Dr. Moewardi General Hospital/Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126 Indonesia&Department of Internal Medicine Dr. Moewardi General Hospital/Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126 Indonesia
2. Department of Internal Medicine Dr. Moewardi General Hospital/Faculty of Medicine, Universitas Sebelas Maret, Surakarta, 57126 Indonesia
- Publication Type:Journal Article
- Keywords:
Sepsis;
NADPH oxidase;
E. elatior.
- From:Tropical Biomedicine
2024;41(No.1):14-19
- CountryMalaysia
- Language:English
-
Abstract:
Infectious diseases with complications of sepsis are still public health concern in both developed and
developing countries. Sepsis is a potentially life-threatening systemic immune response to infection
that can lead to end- stage organ failure and death. Sepsis involves multiple mechanisms such as
neuroendocrine, complement activation, blood coagulation, and fibrinolytic system. Reactive oxygen
species (ROS) is an inflammatory mediator produced by NADPH oxidase activation. This study aimed
to investigate the effects of ethanol extract of E. elatior fruits on NADPH oxidase activity. Forty Mus
musculus mice were randomized divided into five groups (n=8), with the intervention group receiving an
intraperitoneal injection of 0.3 mg/kg BW lipopolysaccharide (LPS). There was a normal group without
LPS injection (N-1), LPS injection only (N-2), and those that received LPS injection and ethanol extracts
of E. elatior fruits containing 2.1 mg/20 g (N-3), 4.2 mg/20 g (N-4), and 8.4 mg/20 g (N-5). NADPH
oxidase activity were measured using ELISA. The oneway ANOVA was used to investigate the differences
between the groups. After administration of the extract at a varied dose, N-5 group the lowest NADPH
oxidase activity (p=0.001). The ethanol extract of E. elatior fruit has antioxidant effects. In this study, a
dose of 8.4 mg/20 g of extract significantly reduced NADPH oxidase activity. The ethanol extract of E.
elatior might be considered a treatment in sepsis.
- Full text:2024071011453678148.2024my1448.pdf
