Experimental study of PD⁃1/PD⁃L1 signal pathway probe targeting osteosarcoma
10.19405/j.cnki.issn1000-1492.2023.11.004
- Author:
Haifeng Huang
1
;
Huan Zou
2
;
Lian Wang
1
;
Xianteng Yang
1
;
Hua Zhu
3
;
Shanshan Li
4
Author Information
1. Dept of Orthopaedics , Guizhou Provincial People ′s Hospital , Guiyang 550002
2. Dept of Rehabilitation , Guizhou Provincial Staf Hospital , Guiyang 550025
3. Dept of Nuclear Medicine , Peking University Cancer Hospital & Institute , Key Laboratory of Carcinogenesis and Translational Research , Ministry of Education/Beijing , Key Laboratory for Research and Evaluation of Radiopharmaceuticals , National Medical Products Administration , Beijing 100142
4. Dept ofAnesthesiology , Guizhou Provincial People ′s Hospital , Guiyang 550002
- Publication Type:Journal Article
- Keywords:
osteosarcoma;
PD⁃1;
PD⁃L1;
PET/CT;
monoclonal antibody;
immune checkpoint
- From:
Acta Universitatis Medicinalis Anhui
2023;58(11):1823-1827
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To explore a non⁃invasive assay for screening osteosarcoma patients with positive programed death receptor⁃1 ( PD⁃1) /programmed death receptor ligand⁃1 ( PD⁃L1) signaling pathway expression
Methods :The subcutaneous tumor bearing mouse model of human Osteosarcoma cells ( OS⁃732) was established by the method of tumor formation , and toxicity test was performed to verify the toxicity of PD⁃L1 antibody to mouse organs . 124 I ⁃anti⁃PD⁃L1 monoclonal antibody molecular probe was further synthesized , and OS⁃732 rats were injected with 18 . 5 MBq 124 I ⁃anti⁃PD⁃L1 probe in tail vein to start OS⁃732 osteosarcoma Micro Positron E mission Tomography/ Computed Tomography (Micro⁃PET/CT) imaging.
Results :OS⁃732 osteosarcoma model was successfully constructed , immunohistochemistry confirmed the presence of PD⁃L1 expression in OS⁃732 osteosarcoma , and Micro⁃PET/CT imaging was successfully performed at different time points (2 , 24 and 48 h after probe injection) to achieve non⁃invasive real⁃time observation of PD⁃L1 in OS⁃732 osteosarcoma .
Conclusion : In this study , the 124 I ⁃anti⁃PD⁃L1 monoclonal antibody molecular probe was constructed in vitro , and Micro PET /CT imaging verified that the probe successfully targeted the PD⁃L1 receptor of OS⁃732 Osteosarcoma , and showed clear immune imaging , indicating that it is hopeful to achieve non⁃invasive screening of Osteosarcoma patients with PD⁃L1 positive expression in clinical practice , and this technology might benefit the majority of Osteosarcoma patients .
- Full text:2024062817282812949靶向骨肉瘤PD-1_PD-L1信号通路探针的实验研究_黄海峰.pdf
