Central projection of GABAergic retinal ganglion cells and their distribution and morphology in retina
10.3760/cma.j.cn115354-20201031-00864
- VernacularTitle:GABA能视网膜神经节细胞的中枢投射区域及其在视网膜中的分布、形态研究
- Author:
Danrui CAI
1
;
Yin SHEN
Author Information
1. 西安交通大学附属第二医院眼科，西安 710000
- Keywords:
Retinal ganglion cell;
γ-aminobutyric acid;
Superior colliculus
- From:
Chinese Journal of Neuromedicine
2021;20(8):765-770
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the central projection of GABAergic retinal ganglion cells (RGCs) and the distribution and morphology of GABAergic RGCs projected to the superior colliculus (SC) in retina.Methods:Six vesicular GABA transporter ( vGAT)- cre transgenic mice (experimental group I) and 6 wide-type C57BL/6 mice (control group I) were given injection of adeno-associated virus rAAV-EF1α-DIO-EGFP-EGFP into the bilateral vitreous chamber; 6 vGAT-cre transgenic mice (experimental group II) and 6 wide type C57BL/6 mice (control group II) accepted injection of adeno-associated virus rAAV-EF1α-DIO-EGFP-EGFP into the bilateral SC using stereotactic technique. Forty-two d after virus injection, 6 mice in the experimental group I, 6 mice in the control group I, 3 mice in the experimental group II, and 3 mice in the control group II were chosen; their retina, optic nerves and brain tissues were cut into slices for double-labelling immunofluorescent staining to detect the green fluorescent protein (GFP) and GABA expressions. Three mice in the experimental group II and 3 mice in the control group II were chosen to prepare stretched preparations of retina; they were performed double-labelling immunofluorescent staining to detect the GFP and BRN3A expressions, count the number of positive cells, and measure the cell body size. Results:In the experimental group I, the green fluorescence signal expressing GFP and red fluorescence signal expressing GABA were co-standardized in the inner plexiform layer, inner nuclear layer and ganglion cell layer of the retina, the green fluorescent signal expressing GFP could be observed in the optic nerves in a linear arrangement along the axon fibers, and green fluorescent signal expressing GFP was observed in the dorsal lateral geniculate nucleus and the superficial SC; while there was no green fluorescent signal in the retina of control group I. In the experimental group II, the green fluorescence signal expressing GFP and red fluorescence signal expressing GABA were co-standardized; the GABAergic RGCs projected to the SC were mainly small/medium-sized cells (diameter 12-16 μm), which was about (37.70±5.33)% of the total cell number and was about (7.27±0.81)% of the ganglion cells (with positive BRN3A).Conclusion:GABAergic RGCs projected to the dorsal lateral geniculate nucleus and SC are specified, and they are small/medium-sized cells and uniformly distributed in the retina.
