Expressions of regulatory B cells in peripheral blood of patients with Guillain-Barre syndrome
10.3760/cma.j.issn.1671-8925.2019.11.013
- VernacularTitle:调节性B细胞及相关细胞因子在吉兰-巴雷综合征患者外周血中的表达
- Author:
Yan WANG
1
;
Chenguang HAO
;
Yanfang LI
;
Yugang ZHENG
;
Jianhua MA
Author Information
1. 新疆医科大学第一附属医院神经内科
- Keywords:
Regulatory B cell;
Guillain-Barre syndrome;
Cytokine;
Interleukin
- From:
Chinese Journal of Neuromedicine
2019;18(11):1151-1154
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expressions of regulatory B cells and related cytokines in patients with Guillain-Barre syndrome (GBS).MethodsForty-four patients with GBS admitted to our hospital from October 2018 to June 2019 were enrolled as GBS group; 44 healthy subjects accepted physical examination in our hospital at the same period were selected as control group. Flow cytometry was used to determine the proportions of CD19+CD24hiCD38hi regulatory B cells and CD19+CD24hiCD27+ regulatory B cells in CD19+ lymphocytes in peripheral blood mononuclear cells of the two groups. ELISA was employed to detect the serum contents of interleukin (IL)-10, IL-35 and transforming growth factor (TGF)-β1 of the two groups.ResultsAs compared with those in the control group, the proportions of CD19+CD24hiCD38hi regulatory B cells and CD19+CD24hiCD27+ regulatory B cells in CD19+B lymphocytes of the GBS group were significantly decreased (P<0.05). As compared with the control group, the GBS group had significantly decreased IL-10 and IL-35 levels (P<0.05), and obviously increased TGF-β1 content without statistical difference (P>0.05).ConclusionRegulatory B cells play a role in the pathogenesis of GBS through IL-10 and IL-35 cytokine pathways.
