Expressions of HAUSP and NANOG in human gliomas and their significance
10.3760/cma.j.issn.1671-8925.2013.01.002
- VernacularTitle:HAUSP与NANOG在胶质瘤中的表达及意义
- Author:
Chuan-Dong CHENG
1
;
Chao-Shi NIU
;
Yang YANG
;
Yang WANG
;
Dong-Xue LI
Author Information
1. 230001合肥,安徽医科大学附属省立医院神经外科,脑功能与脑疾病安徽省重点实验室;230001合肥,安徽省脑立体定向神经外科研究所
- Keywords:
Glioma;
HAUSP;
NANOG;
Cancer stem cell
- From:
Chinese Journal of Neuromedicine
2013;12(1):4-8
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the subcellular location of HAUSP and NANOG in glioma cells and their expressions in glioma tissues,and explore their significance in the pathogenesis of gliomas.Methods Seventy-two glioma samples (19 with WHO graded Ⅱ,25 with WHO graded Ⅲ and 28 with WHO graded Ⅳ),collected in our hospital from January 2010 to November 2010,were chosen in our study; protein expressions of HAUSP and NANOG in the glioma tissues were detected by immunohistochemical staining; the subcellular location of HAUSP and NANOG in human glioma cell line U87 was detected by double immunofluorescence staining.Results The positive rate of HAUSP protein in gliomas with different grades was significantly different (F=15.926,P=0.000); the higher the malignancy grade,the higher the positive rate of HAUSP protein (P＜0.05); the malignancy grade of gliomas was positively correlated with the protein expression of HAUSP (r=0.726,P=0.000).The positive rate of NANOG protein in gliomas with different grades was significantly different (F=14.768,P=0.000);the higher the malignancy grade,the higher the positive rate of NANOG protein (P＜0.05); the malignancy grade ofgliomas was positively correlated with the protein expression of NANOG (r=0.685,P=0.000).Both NANOG and HAUSP expressed in U87,and co-located in the nucleus.Conclusion HAUSP and NANOG play important roles in the pathogenesis of human gliomas and are related to the malignancy; NANOG,as the marker of glioma stem cells in undifferentiated state,plays a vital role in maintaining the cancer stem cells in undifferentiated state through deubiquitinylation of HAUSP.
