Effect of LncRNA XR_378418 on the biological behavior and transcriptome of hepatic stellate cells
10.19405/j.cnki.issn1000-1492.2024.01.020
- Author:
Xue Zhang
1
;
Sen Chen
1
;
Xiaoli Wang
1
;
Qiumei Zhou
2
Author Information
1. Pharmacy Faculty，Anhui University of Chinese Medicine，Hefei 230012
2. Experimental Center of Clinical Research，The First Affiliated Hospital of Anhui University of Chinese Medicine，Hefei 230031
- Publication Type:Journal Article
- Keywords:
long non-coding RNA;
liver fibrosis;
hepatic stellate cells;
high-throughput sequencing
- From:
Acta Universitatis Medicinalis Anhui
2024;59(1):127-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To explore the role of long non-coding RNA XR_378418 (LncRNA XR_378418) in the bi- ological behavior of hepatic stellate cells line JS-1 and to probe the potential molecular mechanism of LncRNA XR _378418 involved in liver fibrosis based on transcriptome sequencing．
Methods :In this study，the recombinant plasmid of pcDNA-LncRNA XR_378418 and control plasmid pcDNA-NC were constructed and transfected into JS-1 cells respectively．Then，the expression level of LncRNA XR_378418 was analyzed by quantitative real-time PCR (RT-qPCR) ．The effect of overexpression of LncRNA XR_378418 on proliferation and migration of JS-1 cells were detected by cell counting kit-8 assay ( CCK-8 ) and scratch assay，respectively． Finally，by high-throughput se- quencing analysis，the effect of XR_378418 on the transcriptomics of JS-1 cells was analyzed.
Results :T-qPCR results showed that the expression level of LncRNA XR_378418 in the overexpression group was significantly higher than that in the control group (P＜0. 05) ．The results of CCK-8 and scratch experiment suggested that the prolifer- ation and migration in the pcDNA-LncRNA XR _ 378418 group significantly increased． Furthermore ，the high- throughput sequencing analysis showed that a total of 248 genes were screened by gene differential analysis ，of which 127 were up-regulated ，and 117 were down-regulated． Gene Ontology ( GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses revealed that LncRNA XR_378418 could regulate cell adhesion，autophagy and Ca2 + signaling，etc．
Conclusion :LncRNA XR_378418 promotes the proliferation and migration of JS-1 cells and affects the expression of genes related to cell adhesion and calcium signaling in JS-1 cells.
- Full text:2024062417261904985LncRNA_XR＿378...胞生物学行为及转录组学影响_张雪.pdf
