Performance of calcium sulfate-based root canal sealers for deciduous teeth
10.12016/j.issn.2096-1456.2024.07.002
- VernacularTitle:硫酸钙基乳牙根管封闭剂的性能研究
- Author:
Xiaxin LI
1
;
Xiaofeng LI
;
Quanli LI
Author Information
1. 安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究重点实验室,安徽合肥(230032)
- Keywords:
calcium sulfate;
chlorhexidine;
root canal sealing;
deciduous teeth;
degradable;
biocompati-ble;
antibacterial properties;
tissue sectioning
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2024;32(7):494-501
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the physicochemical and biological properties of a new calcium sulfate-based root canal sealer for deciduous teeth containing calcium sulfate hemihydrate,barium sulfate,chlorhexidine acetate,and polyethylene glycol 400(PEG 400).Methods This study was reviewed and approved by the Ethics Committee.The calcium sulfate hemihydrate and barium sulfate powders with different mass percentages were mixed with liquid PEG 400 at a powder-to-liquid ratio of 3∶1,and chlorhexidine acetate was added to a concentration of 0.2 mg/mL accord-ing to the volume of PEG 400.The above materials were mechanically ground at 250 r/min for 24 h to obtain a calcium sulfate-based root canal sealer for deciduous teeth.The sealer was classified into different groups according to mass percentages of components.The mass percentages of components were optimized by performing time,fluidity,and radi-opacity experiments,and then the pH,mass loss in vitro,and microscopic morphology of the optimal sealer were evaluat-ed.The antimicrobial properties of the sealer were evaluated by a bacterial-material cocultivation method.The cytocom-patibility of the sealer was evaluated by a CCK-8 assay and cytomorphological staining,and its biocompatibility was evaluated by a subcutaneous tissue embedding assay.Results After optimization,mass percentage of calcium sulfate hemihydrate was 80 wt％,and the mass percentage of barium sulfate was 20 wt％.The flowability and radiopacity of the sealer were in accordance with international standards.The pH stabilized between 6-7.On the 7th and 14th days,the pH in the water group was significantly greater than that in the PBS group(P＜0.001),although the pH in both groups gradually increased(P＞0.05).In vitro degradation experiments,the mass loss of the sealer was approximately 15.17％during the preimmersion period,and rate of mass loss decreased after 3 weeks,reaching only approximately 8.33％.X-ray diffraction(XRD)and scanning electron microscopy(SEM)revealed that the main component of the sealer after hydration was calcium sulfate dehydrate.In bacterial growth assays and cytological tests,the sealer showed significant inhibition of the growth of E.faecalis(P＜0.001).After 1 and 4 days of culture,the cell viability in the 1∶10 and 1∶20 sealer extract dilution group was lower than that in the control group(P＜0.05).On the 7th day,the 1∶20 sealer extract dilution had no significant effect on cell proliferation(P＞0.05).Both the sealer group and the control group(Vitapex and zinc oxide eugenol)caused mild inflammatory reactions in tissue sections.Conclusion In this study,a new type of root canal sealer for deciduous teeth was designed based on calcium sulfate,which has good physicochemical properties and strong antibacterial properties and meets biocompatibility requirements.This study provides an idea for the develop-ment of a new type of root canal sealer for deciduous teeth.
- Full text:2024061710230584419硫酸钙基乳牙根管封闭剂的性能研究.pdf
