Ethanol extract of Abelmoschus manihot suppresses endoplasmic reticulum stress in contrast-induced nephropathy
- Author:
Xin Lin
1
;
Xin Lu
1
;
Yun-He Zhao
1
;
Yi-Bei Wang
1
;
Ru-Ge Niu
1
;
Xiao-Hu Chen
1
Author Information
- Publication Type:Journal Article
- Keywords: Contrast-induced nephropathy; Abelmoschus manihot extract; ER stress; Apoptosis; GRP78; CHOP
- From:Asian Pacific Journal of Tropical Biomedicine 2024;14(1):17-27
- CountryChina
- Language:English
- Abstract: Objective: To explore the efficacy and potential mechanisms of the ethanol extract of Abelmoschus manihot (L.) Medic in contrast-induced nephropathy (CIN). Methods: CIN rat models and human renal proximal tubular cells (HK-2) with iopromide-induced injury were employed to mimic CIN conditions. The effect of Abelmoschus manihot extract on the rat models and HK-2 cells was evaluated. In rat models, kidney function, histology, oxidative stress and apoptosis were determined. In HK-2 cells, cell viability, apoptosis, mitochondrial membrane potential, and endoplasmic reticulum stress were assessed. Results: Abelmoschus manihot extract significantly improved structural and functional impairments in the kidneys of CIN rats. Additionally, the extract effectively mitigated the decline in cellular viability and reduced iopromide-induced oxidative stress and lipid peroxidation. Mechanistic investigations revealed that Abelmoschus manihot extract prominently attenuated acute endoplasmic reticulum stress-mediated apoptosis by downregulating GRP78 and CHOP protein levels. Conclusions: Abelmoschus manihot extract can be used as a promising therapeutic and preventive agent in the treatment of CIN.
- Full text:20240103.pdf
