Research on the Technology for Enzymatic Preparation of Scutellarein
10.19378/j.issn.1003-9783.2024.02.015
- VernacularTitle:酶法制备野黄芩素的工艺研究
- Author:
Yujie CHENG
1
;
Yunhua LIU
;
Zhifang HUANG
;
Yan CHEN
;
Yuhong LIU
;
Jinhai YI
Author Information
1. 成都中医药大学药学院,四川 成都 611130;四川省中医药科学院中药材品质及创新中药研究四川省重点实验室,四川 成都 610041
- Keywords:
Erigeron breviscapus(Vant.)Hand.Mazz.;
scutellarin;
scutellarein;
scutellaria baicalensis stems and leaves glucuronic hydrolase(sbsl GUS);
enzymatic hydrolysis conversion;
technological study
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2024;35(2):274-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective Scutellaria baicalensis stems and leaves glucuronic hydrolase(sbsl GUS)was used to enzymatically hydrolyze scutellarin in Erigeron breviscapus(Vant.)Hand.Mazz.to prepare scutellarein,and the high-purity scutellarein was obtained through separation and purification.Methods Orthogonal experiments were used to optimize the process parameters for the extraction of Erigeron breviscapus(Vant.)Hand.Mazz..Using the rate of enzymatic hydrolysis conversion of scutellarin as the index,the amount of enzyme,pH,temperature,time and antioxidant were investigated,and the preparation process parameters of scutellarein were optimized.Ethanol extraction,activated carbon decolorization,and fractional crystallization were used to purify the crude extract.Results The extraction process was determined to be:segments of Erigeron breviscapus were decocted twice with 10 times water for 1 hour each time.The preparation process of scutellarein was as follows:the amount of sbsl GUS extract and Erigeron breviscapus decoction was 1∶10 based on crude drugs,0.5%sodium metabisulfite was added,pH value was about 6.0,the temperature was about 45℃,and the time was 20 hours.The crude extract of scutellarein with the content more than 60%was obtained.The crude extract was purified by fractional crystallization,refluxed with 80%ethanol,decolorized with activated carbon,concentrated and crystallized,and the scutellarein extract with content more than 85%was obtained.Conclusion sbsl GUS enzymatic hydrolysis technology,which was used to prepare scutellarein,is simple and feasible.This study provides a new way for the manufacture of scutellarein.
