Analysis of amino acid and protein components of commercial swim-bladders
10.3969/j.issn.1001-1528.2024.02.024
- VernacularTitle:市售鱼鳔氨基酸及蛋白质成分分析
- Author:
Jin-Ju ZHANG
1
;
Yi-Hong LU
;
Qing HE
;
Meng-Hua WU
;
Zhi-Guo MA
;
Ying ZHANG
;
Hui CAO
Author Information
1. 暨南大学岭南传统中药研究中心, 广东 广州 510632;国家中药现代化工程技术研究中心岭南资源分中心, 广东 广州 510632
- Keywords:
fish swim-bladder;
amino acid;
protein;
high performance liquid chromatography;
proteomics
- From:
Chinese Traditional Patent Medicine
2024;46(2):499-506
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the amino acids and proteins in 16 batches of commercial fish swim-bladders with different origins.METHODS A high performance liquid chromatography method based on pre-column derivatization using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate(AQC)was developed for the determination of contents and components of 17 amino acids in fish swim-bladders.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)was performed to analyze the molecular weight distribution of proteins from different fish swim-bladders,and proteins in fish swim-bladders were identified by proteomics method.RESULTS The result showed that the determination of 17 amino acids had a good linear relationship(R2≥0.998 0).The average recovery rate was 85.62%-109.60%and the relative standard deviations of precision,stability and repeatability were less than 3.5%.The total content of the 17 amino acids in 16 batches of fish swim-bladders ranged from 468.31 mg/g to 620.05 mg/g.A total of 688 proteins including 11 collagens were identified from 16 batches of fish swim-bladder samples and a plenty of low-abundance proteins at 52-95 kDa were also detected in fish swim-bladders by SDS-PAGE.CONCLUSION This study provides a good reference for the quality evaluation and further utilization of fish swim-bladders.
