Influencing factors in scale-up of extraction process for Yunpi Xiaoshi Prescription
10.3969/j.issn.1001-1528.2024.02.007
- VernacularTitle:运脾消食方提取工艺放大影响因素研究
- Author:
Xin-Rong LIN
1
;
Zi-Wei GAO
;
Ya-Chun SHU
;
Xia ZHAO
;
Lei WU
Author Information
1. 南京中医药大学附属医院, 江苏 南京 210029;南京中医药大学, 江苏 南京 210029
- Keywords:
Yunpi Xiaoshi Prescription;
scale-up of extraction process;
influencing factors;
content determination;
fingerprints;
HPLC
- From:
Chinese Traditional Patent Medicine
2024;46(2):391-396
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the influencing factors in scale-up of extraction process for Yunpi Xiaoshi Prescription.METHODS HPLC was adopted in the content determination of catechin,ferulic acid,taxifolin,isovitexin,narirutin,atractylenolideⅡ,naringin,morin,hesperidin,luteolin,hederagenin,atractylenolideⅠ,naringenin and hesperetin,the fingerprints were established,after which the effects of container volume,optimal fire and feeding quantity on the contents of various constituents were evaluated.RESULTS Fifteen batches of samples demonstrated the similarities of more than 0.995.Fourteen constituents showed good linear relationships within their own ranges(r>0.999 0),whose average recoveries were 96.4%-103.3%with the RSDs of 0.5%-2.7%.The influencing degree of optimal fire was greater than that of container volume and feeding quantity.CONCLUSION The combination of multi-component content determination and fingerprints can provide data basis and theoretical reference for the technology of consistency evaluation in scale-up of extraction process for Yunpi Xiaoshi Prescription.
