Simultaneous content determination of nine constituents and chemical pattern recognition for Fuyang Granules
10.3969/j.issn.1001-1528.2024.02.003
- VernacularTitle:肤痒颗粒中9种成分含量同时测定及其化学模式识别研究
- Author:
Chun-Xia XIAO
1
;
Xiao-Jing HUANG
;
Li XU
;
Ting-Ting LI
;
Ji LI
;
Xiao-Qin ZHAO
Author Information
1. 成都市药品检验研究院, 国家药品监督管理局中药材质量监测评价重点实验室, 四川 成都 610045
- Keywords:
Fuyang Granules;
chemical constituents;
content determination;
UHPLC;
heatmap clustering analysis;
principal component analysis
- From:
Chinese Traditional Patent Medicine
2024;46(2):365-370
- CountryChina
- Language:Chinese
-
Abstract:
AIM To simultaneously determine the contents of neochlorogenic acid,caffeic acid,chlorogenic acid,cryptochlorogenic acid,hydroxysafflor yellow A,ferulic acid,senkyunolide I,senkyunolide H and senkyunolide A in Fuyang Granules,and to make chemical pattern recognition.METHODS The UHPLC was performed on a 35℃thermostatic Waters Acquity UPLC?BEH C18 column(150 mm×2.1 mm,1.7 μm),with the mobile phase comprising of acetonitrile-0.01%phosphoric acid flowing at 0.4 mL/min in a gradient elution manner,and the detection wavelengths were set at 278,322,325,390 nm.Then heatmap clustering analysis and principal component analysis were adopted.RESULTS Nine constituents showed good linear relationships within their own ranges(r>0.999 0),whose average recoveries were 93.89%-102.25%with the RSDs of 0.85%-2.88%.Different batches of samples from the same enterprises demonstrated consistent overall qualities,while the overall qualities of samples from different enterprises exhibited obvious differences.CONCLUSION This simple and accurate method can be used for the quality control of Fuyang Granules.
