Simultaneous content determination of ten constituents in Tianma Toufengling Capsules by QAMS
10.3969/j.issn.1001-1528.2024.02.002
- VernacularTitle:一测多评法同时测定天麻头风灵胶囊中10种成分的含量
- Author:
Xu-Sheng GUO
1
;
Xue-Wei QIU
;
Li LI
;
Ai-Ying WU
;
An-Zhen CHEN
;
Hong-Bing LIU
;
Jing-Guang LU
Author Information
1. 中国海洋大学, 山东 青岛 266000;海洋中药质量研究与评价重点实验室, 山东 青岛 266000
- Keywords:
Tianma Toufengling Capsules;
chemical constituents;
content determination;
quantitative analysis of multi-components by single-marker(QAMS)
- From:
Chinese Traditional Patent Medicine
2024;46(2):359-364
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a quantitative analysis of multi-components by single-marker(QAMS)method for the simultaneous content determination of gastrodin,parishin E,syringin,parishin B,parishin C,ferulic acid,parishin A,buddleoside,harpagoside and cinnamic acid in Tianma Toufengling Capsules.METHODS The analysis was performed on a 30℃thermostatic GL Science InertsilTM ODS-3 column(150 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1%phosphoric acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 220,280 nm.Syringin was used as an internal standard to calculate the relative correction factors of the other nine constituents,after which the content determination was made.RESULTS Ten constituents showed good linear relationships within their own ranges(r≥0.999 7),whose average recoveries were 98.53%-102.22%with the RSDs of 1.26%-2.68%.The result obtained by QAMS approximated those obtained by external standard method.CONCLUSION This accurate and specific method can be used for the quality control of Tianma Toufengling Capsules.
