Extraction process,enzymatic properties and practical application of glucuronic hydrolase in Scutellaria baicalensis stems and leaves
10.3969/j.issn.1001-1528.2024.01.007
- VernacularTitle:黄芩茎叶葡萄糖醛酸水解酶提取工艺、酶学性质、实际应用研究
- Author:
Yu-Jie CHENG
1
;
Xu CHEN
;
Yun-Hua LIU
;
Zhi-Fang HUANG
;
Yan CHEN
;
Yu-Hong LIU
;
Jin-Hai YI
Author Information
1. 成都中医药大学药学院, 四川 成都 611130;四川省中医药科学院, 中药材品质及创新中药研究四川省重点实验室, 四川 成都 610041
- Keywords:
Scutellaria baicalensis stems and leaves;
glucuronic hydrolase;
extraction process;
enzymatic properties;
practical application
- From:
Chinese Traditional Patent Medicine
2024;46(1):35-40
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the extraction process,enzymatic properties and practical application of glucuronic hydrolase in Scutellaria baicalensis stems and leaves(sbsl GUS).METHODS With granularity,water consumption,extraction time and extraction frequency as influencing factors,enzymatic activity as an evaluation index,the extraction process was optimized by orthogonal test on the basis of single factor test.The relationship between substrate(baicalin)concentration and enzymolysis rate,after which Vmax and Km were calculated,the effects of pH value,temperature and metal ion on enzymatic activity were investigated,pH stability and heat stability were evaluated.sbsl GUS was adotped in the enzymolysis of baicalin to prepare baicalein,then the effects of pH value,temperature,reaction time,initial substrate concentration and enzyme addition on transfer rate were investigated.RESULTS The optimal extraction process was determined to be 40 mesh for granularity,10 times for water consumption,15 min for extraction time,and 3 times for extraction frequency.The enzymolysis accorded with the kinetics of enzymatic reaction,Km was 0.006 3 mol/L,Vmax was 70.42 μmol/h,the strongest enzymatic activity was found at the pH value of 6.0,temperature of 45℃and metal ion of 100 mmol/L Cu2+,sbsl GUS demonstrated good stability at the ranges of 4.0-7.0 for pH value and 4-30℃for temperature.The optimal preparation process was determined to be 6.0 for pH value,45℃for temperature,more than 12 h for reaction time,67.2 mmol/L for initial substrate concentration,and 1 mL/0.269 mmol baicalin for enzyme addition,the transfer rate was 97.83%.CONCLUSION sbsl GUS enzymolysis exhibits high efficiency and mild condition,which can provide a simple preparation method for obtaining baicalein,and expand the application path of Scutellaria baicalensis stems and leaves.
