Simultaneous content determination of six constituents in Gaultheria leucocarpa var.yunnanensis by UPLC-MS/MS
10.3969/j.issn.1001-1528.2024.01.005
- VernacularTitle:UPLC-MS/MS法同时测定透骨香中6种成分的含量
- Author:
Lin JIN
1
;
Zu-Ying ZHOU
;
Jia-Hua ZHOU
;
Ming-Yan CHI
;
Lin ZHENG
;
Yong HUANG
;
Wen HUANG
Author Information
1. 贵州医科大学, 贵州省药物制剂重点实验室/药用植物功效与利用国家重点实验室, 贵州 贵阳 550004;贵州医科大学药学院, 贵州 贵阳 550004
- Keywords:
Gaultheria leucocarpa var.yunnanensis;
chemical constituents;
content determination;
UPLC-MS/MS;
hierarchical cluster analysis(HCA);
principal component analysis(PCA)
- From:
Chinese Traditional Patent Medicine
2024;46(1):22-28
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an UPLC-MS/MS method for simultaneous content determination of protocatechuic acid,epicatechin,chlorogenic acid,quercitrin,gaultherin and gaultheroside A in Gaultheria leucocarpa var.yunnanensis.METHODS The analysis was performed on a 40℃thermostatic Waters BEH C18 column(100 mm×2.1 mm,1.7 μm),with the mobile phase comprising of water(containing 0.1%formic acid)-acetonitrile(containing 0.1%formic acid)flowing at 0.3 mL/min in a gradient elution manner,and electron spray inoization source was adopted in positive and negative ion scanning with multiple reaction monitoring(MRM)mode.Hierarchical cluster analysis(HCA)and principal component analysis(PCA)was used to screen important components that affect the quality of medicinal materials.RESULTS Six constituents showed good linear relationships within their own ranges(R2≥0.998 2),whose average recoveries were 98.76%-101.88%with the RSDs of 1.0%-2.5%.The constituents of G.leucocarpa in the roots and aerial parts were quite different.Gaultherin,epicatechin and protocatechuic acid may be the quality mark constituents of G.leucocarpa.CONCLUSION This accurate and efficient method can be used for the quality control of G.leucocarpa.
