Simultaneous content determination of ten constituents in Xiao'er Ganmao Granules by HPLC
10.3969/j.issn.1001-1528.2024.01.002
- VernacularTitle:HPLC法同时测定小儿感冒颗粒中10种成分的含量
- Author:
Dong-Fang ZHANG
1
;
Xiao-Guang LU
Author Information
1. 南阳市产品质量检验检测中心, 河南 南阳 473059
- Keywords:
Xiao'er Ganmao Granules;
chemical constituents;
content determination;
HPLC
- From:
Chinese Traditional Patent Medicine
2024;46(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of urine,guanosine,adenosine,(R,S)-hysterone,chlorogenic acid,forsythian glycoside A,luteolin,3,5-dicaffeioyl quinic acid,4,5-dicaffeioyl quinic acid and phillyrin in Xiao'er Ganmao Granules.METHODS The analysis was performed on a 40℃thermostatic Welchrom C18 column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of acetonitrile-0.2%glacial acetic acid flowing at 0.8,1.0 mL/min in a gradient elution manner,and the detection wavelengths were set at 230,254,327 nm.RESULTS Ten constituents showed good linear relationships within their own ranges(r≥0.999 0),whose average recoveries were 93.68%-98.08%with the RSDs of 0.90%-1.86%.CONCLUSION This stable and reliable method can be used for the quality control of Xiao'er Ganmao Granules.
