Simultaneous determination of five constituents in Artemisia sieversiana and Ajania tenuifolia by HPLC
10.3969/j.issn.1001-1528.2023.12.024
- VernacularTitle:HPLC法同时测定大籽蒿和细叶亚菊中5种成分
- Author:
Yu-Chen XIE
1
;
Fang PENG
;
Yue WANG
;
Huan YI
;
Xin-Mei XU
;
Gang FAN
Author Information
1. 成都中医药大学药学院,四川 成都 611137
- Keywords:
Artemisia sieversiana Ehrhart ex Willd.;
Ajania tenuifolia(Jacquem.ex DC.)Tzvelev in Schischk.&Bobrov;
chemical constituents;
HPLC
- From:
Chinese Traditional Patent Medicine
2023;45(12):4000-4004
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an UPLC-MS/MS method for the simultaneous content determination of chlorogenic acid,umbellifolactone,rutin,isochlorogenic acid A and isochlorogenic acid C in Artemisia sieversiana Ehrhart ex Willd.and Ajania tenuifolia(Jacquem.ex DC.)Tzvelev in Schischk.&Bobrov by HPLC,and compare the differences between them.METHODS The analysis of 50%methanol extract of both was performed on a 30℃thermostatic CAPCELL PAK-C18 column(100 mm×2.1 mm,1.7 μm),with the mobile phase comprising of acetonitrile-0.2%phosphoric acid water,flowing at 1 mL/min in a gradient elution manner,and the detection wavelengths were set at 290 nm.A t-test was used to compare the contents differences of five constituents in the two crude drugs.RESULTS Five constituents showed good linear relationships within their own ranges(r≥0.999 5),whose average recoveries were 96.03%-103.11%,with the RSDs of 0.51%-2.26%.The average contents of chlorogenic acid,umbellifolactone,rutin,isochlorogenic acid A and isochlorogenic acid C in A.sieversiana and A.tenuifolia were 3.61,0.71,1.46,8.74,1.44 mg/g and 2.60,0.26,0.79,6.31,1.22 mg/g,respectively.CONCLUSION The method is accurate,reliable and reproducible.There was no significant difference in the five chemical constituents between A.sieversiana and A.tenuifolia.
