Effect of dexmedetomidine on proteomics during intestinal barrier injury in burned rats
10.3760/cma.j.cn131073.20230401.00820
- VernacularTitle:右美托咪定对烧伤大鼠肠道屏障损伤时蛋白质组学的影响
- Author:
Chao QIN
1
;
Ning LUO
;
Qingqing HAN
;
Yonghao YU
Author Information
1. 天津医科大学总医院麻醉科 天津市麻醉学研究所，天津 300052
- Keywords:
Dexmedetomidine;
Burns;
Intestinal mucosa;
Proteomics
- From:
Chinese Journal of Anesthesiology
2023;43(8):996-1001
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the effects of dexmedetomidine on proteomics during intestinal barrier injury in burned rats.Methods:Sixty-four clean-grade healthy adult male Sprague-Dawley rats, aged 8-12 weeks, weighing 250-300 g, were divided into 4 groups ( n=16 each) by the random number table method: sham operation group (Sham group), sham operation plus dexmedetomidine group (Sham-Dex group), burn group (Burn group) and burn plus dexmedetomidine group (Burn-Dex group). Rats were subjected to Ⅲ degree burn covering 40% of the total body surface area on back in Burn group and Burn-Dex group, and the back hair was removed and then the rats were exposed to skin temperature with a perm in Sham group and Sham-Dex group. Dexmedetomidine 5 μg·kg -1·h -1 was intravenously infused for 3 h after developing the model in Sham-Dex group and Burn-Dex group. The equal volume of 0.9% normal saline was continuously infused in Sham group and Burn group. Blood from the abdominal aorta was collected at 6 h after stopping administration, then the rats were sacrificed, and intestinal tissues were obtained for examination of pathological changes (HE staining method) and for determination of plasma fluoresce in isothiocyanate-dextran and diamine oxidase (DAO) concentrations. Intestinal damage was assessed and scored according to Chiu. The relative and absolute quantitative isotope labeling and LC-tandem mass spectrometry were performed to screen the differentially expressed proteins. The differentially expressed proteins were analyzed by STRING protein interaction network analysis, gene ontology analysis and KEGG pathway analysis. Results:Compared with Sham group, the Chiu′s score after burn and plasma concentrations of FITC-Dextran and DAO at 6 h after stopping administration were significantly increased in Burn and Burn-Dex groups ( P<0.05). Compared with Burn group, the Chiu′s score after burn and plasma concentrations of FITC-Dextran and DAO at 6 h after stopping administration were significantly decreased in Burn-Dex group ( P<0.05). Analysis of STRING protein interaction network, ontology analysis analysis and KEGG pathway analysis found that the protein genes met the conditions were Psmb10, Psmb7, RGD1310507 and LOC100909441, there was an interaction between Psmb10 and Psmb7, and it was significantly enriched in various pathways such as proteasome. Conclusions:Dexmedetomidine can cause changes in protein expression during intestinal barrier injury in burned rats, and Psmb10 and Psmb7 may be the two protein targets of dexmedetomidine.
