The research of porcine improved perfusion-acellular pancreatic bioscaffold as a natural 3D platform in vitro
10.3760/cma.j.cn113884-20230716-00203
- VernacularTitle:改良灌注法猪全胰腺脱细胞基质支架体外功能的实验研究
- Author:
Xiaolei ZHOU
1
;
Xin WANG
Author Information
1. 天津医科大学总医院普通外科，天津 300052
- Keywords:
Pancreas;
Acellular pancreatic bioscaffold;
Perfusion;
Bone mesenchymal stem cells
- From:
Chinese Journal of Hepatobiliary Surgery
2023;29(11):851-856
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the biochemical properties of porcine improved perfusion-acellular pancreatic bioscaffold (APB).Methods:The fresh pancreas from 10 health pigs [age: 4-6 months, weight: (20.0±2.5) kg] were perfused through portal vein and pancreatic duct by our Easy-Load Digital Drive peristaltic pumps. The histological structure, extracellular matrix (ECM) composition and DNA quantification of APB were evaluated. The biocompatibility of APB was evaluated by transplantation experiment. Also, we observed the proliferation and differentiation of bone mesenchymal stem cells (BMSCs) reseeded on the APB.Results:The pancreatic tissue became translucent and the macroscopic three-dimensional architectures of native pancreas were retained after decellularization. The histological evaluation indicated that the ECM ultrastructure was preserved, and there was no cellular components retained. Compared with the native pancreas, the major ECM components didn’t show significant difference [collagen: (846.1±280.0) ng/mg dry weight vs (754.7±249.6) ng/mg dry weight; sGAG: (433.6±142.6) ng/mg dry weight vs (397.2±98.7) ng/mg dry weight; both P>0.05]. The DNA content of APB decreased significantly compared with native pancreas [(83 464.2±27 813.4) ng/mg dry weight vs (298.9±96.8) ng/mg dry weight, t=11.56, P<0.001]. The histological analysis of the subcutaneous implantation site showed the enhanced histological remold score and benefit remodeling results with time after implantation. The proliferation and differentiation of BMSCs could be promoted on the recellularized APB. Conclusion:Our APB in this study meets the stringent requirement to define a successful decellularization, which could allow APB preserving 3D architecture and represented a biocompatible scaffold capable of supporting and enhancing cell proliferation and differentiation.
