Inhibitory effect of (-)-epigallocatechin gallate on titanium particle-induced TNF-alpha release and in vivo osteolysis.
10.3858/emm.2011.43.7.045
- Author:
Shan JIN
1
;
Ju Young PARK
;
Jung Min HONG
;
Tae Ho KIM
;
Hong In SHIN
;
Eui Kyun PARK
;
Shin Yoon KIM
Author Information
1. Skeletal Diseases Genome Research Center, Kyungpook National University Hospital, Kyungpook National University, Daegu 700-412, Korea. epark@knu.ac.kr, syukim@knu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
epigallocatechin gallate;
mitogen-activated protein kinases;
transcription factor AP-1;
NF-kappaB, titanium;
tumor necrosis factor-alpha
- MeSH:
Animals;
Catechin/*analogs & derivatives/pharmacology;
Cell Line;
Implants, Experimental;
Macrophages/drug effects/metabolism;
Male;
Mice;
Mice, Inbred C57BL;
Mitogen-Activated Protein Kinase 8/metabolism;
NF-kappa B/metabolism;
Osteolysis/chemically induced/*metabolism/prevention & control;
Particulate Matter/*adverse effects;
Prosthesis Failure;
Signal Transduction/drug effects;
Skull/*drug effects/pathology;
Titanium/*adverse effects;
Transcription Factor AP-1/metabolism;
Tumor Necrosis Factor-alpha/*metabolism
- From:Experimental & Molecular Medicine
2011;43(7):411-418
- CountryRepublic of Korea
- Language:English
-
Abstract:
Tumor necrosis factor-alpha (TNF-alpha) and inflammatory cytokines released from activated macrophages in response to particulate debris greatly impact periprosthetic bone loss and consequent implant failure. In the present study, we found that a major polyphenolic component of green tea, (-)-epigallocatechin gallate (EGCG), inhibited Ti particle-induced TNF-alpha release in macrophages in vitro and calvarial osteolysis in vivo. The Ti stimulation of macrophages released TNF-alpha in a dose- and time-dependent manner, and EGCG substantially suppressed Ti particle-induced TNF-alpha release. Analysis of signaling pathway showed that EGCG inhibited the Ti-induced c-Jun N-terminus kinase (JNK) activation and inhibitory kappaB (IkappaB) degradation, and consequently the Ti-induced transcriptional activation of AP-1 and NF-kappaB. In a mouse calvarial osteolysis model, EGCG inhibited Ti particle-induced osteolysis in vivo by suppressing TNF-alpha expression and osteoclast formation. Therefore, EGCG may be a potential candidate compound for osteolysis prevention and treatment as well as aseptic loosening after total replacement arthroplasty.
