The effect of LncRNA-MIAT mediated miR-205 expression on acute kidney injury in septic rats
10.3760/cma.j.cn431274-20230423-00543
- VernacularTitle:LncRNA-MIAT介导miR-205表达对脓毒症大鼠急性肾损伤的影响
- Author:
Yanyan ZHANG
1
;
Yun ZHANG
;
Fei XIA
Author Information
1. 南京医科大学附属苏州医院重症医学科，苏州 215008
- Keywords:
RNA, long noncoding;
miRNA-205;
Sepsis;
Acute kidney injury
- From:
Journal of Chinese Physician
2023;25(12):1845-1848
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of long chain non coding RNA (LncRNA)-myocardial infarction associated transcript (MIAT) mediated expression of microRNA-205 (miR-205) on acute kidney injury in septic rats.Methods:A total of 32 clean grade Sprague-Dawley (SD) rats were randomly divided into a normal group, a model group, a transfection control group, and a transfection group, with 8 rats in each group. Except for the normal group, other groups of rats were used to establish sepsis acute kidney injury models by cecal ligation and puncture method. The control group was transfected with non-specific small interfering RNA (siRNA), while the transfection group was transfected with LncRNA-MIAT specific siRNA. The expression of miR-205 in renal tissue was measured using real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) method; The serum levels of creatinine (Scr) and urea nitrogen (BUN) were measured using a fully automated biochemical analyzer; The levels of serum interleukin-6 (IL-6) and tumor necrosis factor -α (TNF -α) were measured using enzyme-linked immunosorbent assay (ELISA); In situ end labeling (TUNEL) method was used to determine apoptosis in rat kidney tissue cells.Results:Compared with the normal group, the model group, transfected control group, and transfected group had higher relative expression levels of miR-205 (all P<0.05); The relative expression level of miR-205 in the transfection group was lower than that in the model group and transfection control group (all P<0.05). Compared with the normal group, the model group, transfection control group, and transfection group had higher serum Scr and BUN levels (all P<0.05); The serum Scr and BUN levels in the transfection group were lower than those in the model group and transfection control group (all P<0.05). Compared with the normal group, the model group, transfection control group, and transfection group had higher levels of serum IL-6 and TNF -α (all P<0.05); The serum IL-6 and TNF-α levels in the transfection group were lower than those in the model group and the transfection control group (all P<0.05). Compared with the normal group, the model group, transfection control group, and transfection group had higher rates of renal tissue cell apoptosis (all P<0.05); The apoptosis rate of renal tissue cells in the transfection group was lower than that in the model group and transfection control group (all P<0.05). Conclusions:LncRNA-MIAT exerts a renal protective effect on acute kidney injury in septic rats by regulating the expression of miR-205. Its mechanism may be related to downregulating miR-205 expression, reducing inflammatory response, and reducing cell apoptosis in renal tissue.
