The effect of matrix metalloproteinase-8 protein on proliferation and migration of squamous cell carcinoma EC109 cells of the esophagus
10.3760/cma.j.cn431274-20221120-01204
- VernacularTitle:基质金属蛋白酶-8对食管鳞癌EC109细胞增殖和迁移的影响
- Author:
Detian JI
1
;
Hui WEN
;
Xiaobo LIU
;
Qiang TONG
Author Information
1. 十堰市太和医院(湖北医药学院附属医院)心功能室，十堰 442000
- Keywords:
Matrix metalloproteinase 8;
Esophageal squamous cell carcinoma;
Cell proliferation;
Cell migration
- From:
Journal of Chinese Physician
2023;25(10):1496-1501
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of matrix metalloproteinase-8 (MMP-8) on the proliferation and migration of esophageal squamous cell carcinoma EC109 cells, and to explore its possible mechanism.Methods:Esophageal epithelial cells Het-1A and esophageal squamous cell carcinoma cell lines (EC109, CEC2, KYSE150, TE-1 cells) were cultured. Using polymerase chain reaction (PCR) experiments, MMP-8 low expression cell line EC109 cells were screened for plasmid transfection. The MMP-8 overexpression plasmid was MMP-8 overexpression group, and the empty plasmid was the empty vector group. Flow cytometry was used to screen and amplify the transfected cells, and stable transfected cells were constructed. The changes of proliferation and migration were detected by methyl thiazolyl tetrazolium (MTT) and scratch test, and the mRNA expression of proliferation and migration related genes was detected by quantitative real-time polymerase chain reaction (qRT-PCR).Results:MMP-8 expression was lowest in EC109 cells among all esophageal squamous cell carcinoma cell lines. Compared with the empty vector group, the proliferation and migration ability of MMP-8 overexpression group cells were significantly enhanced, and the mRNA expression levels of proliferation and migration related genes AKT1, RAC1, p53, epidermal growth factor receptor (EGFR), MTA1 and RARRES1 were significantly increased (all P<0.05). Conclusions:MMP-8 protein can enhance the proliferation and migration ability of esophageal squamous cell carcinoma EC109 cells. MMP-8 overexpression can promote the mRNA expression of proliferation and migration related genes, which may be related to its promotion of EC109 cell proliferation and migration.
