PPAR-γ inhibits the proliferation of human large cell lung cancer NCI-H460 cells through the NLRP3 inflammasome in a high-glucose microenvironment
10.12007/j.issn.0258-4646.2023.12.002
- VernacularTitle:PPAR-γ抑制高糖微环境下NCI-H460细胞增殖的分子机制
- Author:
Mingliang HU
1
Author Information
1. 中国医科大学附属盛京医院胃肠营养外科,沈阳 110004
- Keywords:
PPAR-γ;
NLRP3 inflammasome;
high-glucose microenvironment;
proliferation;
human large cell lung cancer
- From:
Journal of China Medical University
2023;52(12):1062-1067
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of PPAR-γon the proliferation of human large cell lung cancer(NCI-H460)cells in a high-glucose microenvironment and to explore the associated molecular mechanism.Methods NCI-H460 cells were treated with normal-glucose(blank group),hypertonic(control group),and high-glucose(30 mmol/L;high-glucose group)media.The effects of a high-glucose microenvironment on the proliferation of NCI-H460 cells were analyzed using Cell Counting Kit 8(CCK-8)and colony-for-mation assays.The specific PPAR-γactivator,rosiglitazone,was used to treat NCI-H460 cells in a high-glucose microenvironment,and the expression of NLRP3-inflammasome-related proteins was detected by Western blotting.The effect of PPAR-γon the proliferation of NCI-H460 cells in a high-glucose microenvironment was analyzed by CCK-8 and clony-formation assays.The NLRP3 inflammasome agonist,NSS,combined with rosiglitazone,were used to treat NCI-H460 cells in a high-glucose microenvironment.CCK-8 and clony-formation assays were used to analyze whether the NLRP3 inflammasome was involved in the inhibitory effect of PPAR-γon the proliferation of NCI-H460 cells in a high-glucose microenvironment.Results A high-glucose microenvironment significantly induced the proliferation of NCI-H460 cells,increased the activity of the NLRP3 inflammasome,and reduced the expression levels of PPAR-γprotein.Rosiglitazone effectively inhibited NLRP3 inflammasome activity and NCI-H460 cell proliferation,and this effect was reversed by NLRP3 inflammasome agonist.Conclusion PPAR-γinhibits the proliferation of NCI-H460 cells in a high-glucose microenvironment by down-regulating the activity of the NLRP3 inflammasome.
