Inhibitory effect of miR-133a on liver cancer through tar-geted regulation of G6PD expression
10.3969/j.issn.1009-9905.2024.01.005
- VernacularTitle:miR-133a通过靶向调控G6PD的表达对肝癌的抑制作用
- Author:
Ya-Dong WANG
1
;
Xue-Jun SUN
;
Chun-Yu YANG
;
Gui-Ping WANG
;
Ming JIN
;
He LI
;
Jia-Jun YIN
Author Information
1. 大连大学附属中山医院 普通外科(辽宁 大连 116001)
- Keywords:
miR-133a;
G6PD;
Hepatocellular,carcinoma
- From:
Chinese Journal of Current Advances in General Surgery
2024;27(1):25-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore if miR-133a is involved in the occurrence and development of hepatocellular carcinoma(HCC)via regulating G6PD.Methods:Bioinformatics analysis predicted the binding sites of miR-133a and G6PD;RT-PCR or western blot was used to assess the expres-sion of miR-133a and G6PD in HCC tissues and the adjacent normal tissues;CCK-8 and flow cy-tometry assays were performed to evaluate the effects of miR-133a/G6PD on cell proliferation,apop-tosis;Fluorescent reporter gene and western blot assays were used to assess the effect of miR-133a on G6PD expression.Results:miR-133a expression was decreased in HCC tissues while G6PD was increased(P0.01);Up-regulation of miR-133a significantly reduced G6PD expression(P＜0.01);up-reg-ulation of miR-133a inhibited cell growth and promoted cell apoptosis(P＜0.05),whereas these effects induced by miR-133a over-expression were all abolished when G6PD was up-regulated(P＜0.01).Conclusion:miR-133a represses the occurrence and development of HCC via targeting G6PD.
