Etiological diagnosis and molecular tracing analysis in a case of imported melioidosis
10.3969/j.issn.1002-2694.2024.00.009
- VernacularTitle:1例输入性类鼻疽的病原学诊断及溯源分析
- Author:
Hong-Xia YANG
1
;
Chun-Yu WANG
;
Yang WANG
;
Rui-E HAO
;
Qi-Yu ZHAO
;
Xiao ZHENG
Author Information
1. 山西省疾病预防控制中心,重点传染性疾病预防与诊治山西省重点实验室,太原 030012
- Keywords:
melioidosis;
Burkholderia pseudomallei;
MLST;
whole gene sequencing;
molecular tracing
- From:
Chinese Journal of Zoonoses
2024;40(1):76-81
- CountryChina
- Language:Chinese
-
Abstract:
We aimed to identify the infectious source of a case of melioidosis,to provide evidence for the prevention and control of melioidosis in Shanxi Province,China.The patient developed repeated fever,fatigue,diarrhea,and other symptoms after being caught in the rain while traveling in Hainan Province.The blood culture was positive,and the bacterial strain was i-dentified as Burkholderia thayensis and sent to the provincial Center for Disease Control and Prevention for further evaluation.MALDI-TOF MS and biochemical identification were used to identify the strain,whole genome sequencing was performed after nucleic acid extraction,MLST type and drug-resistance genes were analyzed,and a phylogenetic tree was constructed.The iso-lated strain was identified as Burkholderia pseudomallei by MALDI-TOF MS and biochemistry,and the MLST type was 366.The whole gene sequencing analysis indicated a close evolutionary relationship with the three isolates in Hainan Province,with high homology.This case of melioidosis was indeed imported from Hainan Province,according to molecular tracing analysis and epidemiological investigation,thus suggesting that medical institutions and disease control departments should strengthen understanding of melioidosis,and improve the diagnosis and treatment ability.
