Mechanism of Fucoidan Phlorofucofuroeckol A on Inhibit the Proliferation and Invasion of Colorectal Cancer Cells by Down-regulating TGF-β1/Smads Signaling Pathway
10.3870/j.issn.1004-0781.2024.04.004
- VernacularTitle:褐藻聚合酚下调TGF-β1/Smads信号通路抑制大肠癌细胞增殖与侵袭
- Author:
Hong LI
1
;
Wei DONG
;
Jie HOU
;
De HE
Author Information
1. 深圳市宝安区人民医院普通外科,深圳 518000
- Keywords:
Phlorofucofuroeckol A;
Colorectal cancer;
Proliferation and invasion;
Transforming growth factor-β1/moth-ers against decapentaplegic homolog 2/3 signaling pathway
- From:
Herald of Medicine
2024;43(4):495-501
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of phlorofucofuroeckol A(PFFE-A)on the proliferation and invasion of colorectal carcinoma cells and its regulation of transforming growth factor-β1(TGF-β1)and mothers against decapentaplegic hom-olog 2/3(Smad2/3)signaling pathway.Methods The cells were processed as follows:the cells were intervened with low,medium and high doses of 50,100,and 150 μmol·L-1 of PFFE-A,respectively and cells in the normal control group were also established.5-Ethynyl-2'-deoxyuridine(EdU)staining was used to detect the cell proliferation.The transwell chamber was used to detect the invasion ability.A xenograft colon cancer nude mice model was used to detect the growth and metastasis ability of the cells in vivo.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of epithelial-to-mes-enchymal transition(EMT)related genes.Western blotting was used to detect the expression levels of TGF-β1 and p-Smad2/3 in cells.Results Compared with normal control group,the proliferation rate,the number of invaded cells,the tumor mass,the pro-portion of tumor metastasis,the expression of N-cadherin mRNA,the expression of TGF-β1 and p-Smad2/3 were significantly de-creased(P＜0.05),and the mRNA expression of E-cadherin was significantly increased(P＜0.05).All were presented with a sig-nificant dose-dependent(P＜0.05).Conclusion PFFE-A could inhibit the EMT process of tumor cells,inhibit the prolifera-tion and invasion of HT29 cells in vitro,and down-regulate the growth and metastasis of HT29 cells in vivo,which may be achieved by down-regulating TGF-β1/Smad2/3 signaling pathway.
