Effects of the Combination of Herba Epimedii Combined with Fructus Ligustri Lucidi on Autophagy Activation State of ASMCs
10.11969/j.issn.1673-548X.2023.12.009
- VernacularTitle:淫羊藿与女贞子配伍对自噬激活状态ASMCs的影响
- Author:
Han WANG
1
;
Zitong MA
;
Yuting LONG
Author Information
1. 100069 首都医科大学中医药学院、中医络病研究北京市重点实验室
- Keywords:
ASMCs;
Herba epimedii/Fructus ligustri lucidi;
Autophagy;
Apoptosis;
Proliferation
- From:
Journal of Medical Research
2023;52(12):36-40,70
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the regulatory effect of herba epimedii(HE)combined with fructus ligustri lucidi(FLL)on auto-phagy,apoptosis and proliferation activity of airway smooth muscle cells(ASMCs)induced by rapamycin(RAP).Methods Primary ASMCs of rats were cultured and randomly divided into five groups:normal group,RAP group,HE group,FLL group and compatible group.Normal rat serum or medicated rat serum was added separately according to the group.Except for normal group,all other groups were simultaneously added with RAP-induced ASMCs,which were examined after 48h.Transmission electron microscope was used to observe cell microstructure;MTT assay was used to detect cell viability;immunocytochemistry was used to detect protein expression of proliferation protein ki-67 to evaluate the proliferation activity of cells;Annexin V-FITC/P1 assay was used to detect the apoptosis ac-tivity of cells;Western blot and cell immunofluorescence were used to detect the protein expressions of LC3,Beclin-1,mTOR,p-mTOR,Bax,Bcl-2,caspase-3 and p53.Results The compatibility of HE and FLL significantly inhibited RAP-induced autophagy in ASMCs,and down-regulated LC3-Ⅱ/Ⅰ protein ratio(P＜0.05)and Beclin-1 protein expression(P＜0.01),alleviated the re-duction of cell viability and proliferation activity(all P＜0.01),and depressed apoptosis activity(P＜0.01).The above effects of com-patibility of HE and FLL were better than those of HE and FLL alone(P＜0.05,P＜0.01).In addition,the compatible group could up-regulate the expression of caspase-3 protein(P＜0.05)and down-regulate the expression of p53 protein(P＜0.01);However,there was no significant effect on the mTOR,p-mTOR,Bax and Bcl-2 protein.Conclusion The compatibility of HE and FLL could suppress the excessive autophagy/apoptosis level of RAP-induced ASMCs,restore the cell viability and proliferation activity of ASMCs,and avoid excessive damage of ASMCs.
