LINC00115 Targets miR-874-3p to Regulate the Biological Behavior and Paclitaxel Sensitivity of Liver Cancer Cells
10.3870/j.issn.1672-0741.23.06.012
- VernacularTitle:LINC00115靶向miR-874-3p调控肝癌细胞生物学行为以及紫杉醇敏感性
- Author:
Wei WANG
1
;
Hui XIA
;
Cheng ZHOU
Author Information
1. 华中科技大学同济医学院附属武汉中西医结合医院,武汉市第一医院肝胆外科,武汉 430022
- Keywords:
liver cancer;
LINC00115;
miR-874-3p;
proliferation;
migration;
invasion;
paclitaxel sensitivity
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2024;53(1):75-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of LINC00115 targeting miR-874-3p on the biological behavior and paclitaxel sensitivity of liver cancer cells.Methods The qRT-PCR was performed to detect the expression levels of LINC00115 and miR-874-3p in liver cancer tissues and cell lines.The si-NC,si-LINC00115,miR-NC,miR-874-3p,pcDNA,pcDNA-LINC00115,anti-miR-NC+si-LINC00115,anti-miR-874-3p+si-LINC00115 were transfected into liver cancer cells MHCC97H respective-ly.CCK-8 method was applied to assess cell viability and IC50 value to paclitaxel.Transwell assay was performed to detect cell migration and invasion.Dual luciferase reporter gene method was used to determine the relationship between LINC00115 and miR-874-3p.Results LINC00115 was highly expressed(P＜0.05),while miR-874-3p was lowly expressed(P＜0.05)in liver cancer tissues and cell lines.After downregulating LINC00115,the cell absorbance(A)value,the IC50 value to paclitaxel,migra-tion and invasion were significantly reduced(all P＜0.05),while miR-874-3p expression was significantly increased(P＜0.05).After upregulating miR-874-3p,the cell A value,IC50 value to paclitaxel,migration and invasion were significantly reduced(all P＜0.05).After upregulating LINC00115,miR-874-3p expression was decreased(P＜0.05).LINC00115 had a direct interaction with miR-874-3p.Downregulating miR-874-3p significantly reduced the effect of low LINC00115 expression on A value,IC50 value to paclitaxel,migration and invasion of liver cancer cells(all P＜0.05).Conclusion Downregulation of LINC00115 inhibits the prolifera-tion,migration and invasion of liver cancer cells to increase paclitaxel sensitivity by promoting miR-874-3p expression.
