circRAF1 regulates the proliferation and apoptosis of human ovarian granulosa cells
10.3969/j.issn.1006-5725.2024.07.006
- VernacularTitle:circRAF1调节人卵巢颗粒细胞的增殖与凋亡
- Author:
Wenxin LI
1
;
Minjun LU
;
Li LIN
;
Yueqin LIU
;
Xiaolan ZHU
Author Information
1. 江苏大学第四附属医院(镇江市妇幼保健院)生殖医学中心,中心实验室(江苏镇江 212001)
- Keywords:
circRAF1;
POI;
GCs;
ovarian reserve;
cell proliferation and apoptosis
- From:
The Journal of Practical Medicine
2024;40(7):910-917,923
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of circRAF1 in primary ovarian insufficiency(POI)and explore its effect on cell proliferation and apoptosis of human ovarian granulosa cells(GCs)line(KGN cells).Methods The expression of circRAF1 in GCs and serum of patients with normal ovarian reserve function(n = 50)and patients with POI(n = 50)were detected with RT-qPCR.The correlation of circRAF1 with ovarian reserve function indexes was analyzed.Small interfering RNA(siRNA)targeting circRAF1 was constructed and trans-fected into KGN cells,with the cell proliferation detected by CCK-8 and EdU assay,and the cell apoptosis detected by JC-1 and Tunel assay.The mRNA and protein levels of genes related to cell proliferation and apoptosis(FSHR,PCNA,Bcl-2,Casp-9,Bax)were detected by RT-qPCR and WB.Results The expression of circRAF1 decreased in GCs and serum of POI patients.The expression of circRAF1 was positively correlated with serum E2 and AMH levels(P<0.001),but negatively correlated with serum FSH and LH levels(P<0.001).At the same time,the expression of circRAF1 was positively correlated with AFC(P<0.001).Interfering with the expression of circRAF1 could inhibit the proliferation of KGN cells and promote their apoptosis.Conclusion The expression of circRAF1 in the GCs and serum of POI patients is down-regulated,which is correlated with the decline of ovarian reserve function.Interfering with circRAF1 can inhibit the proliferation of GCs and promote their apoptosis.
