Correlation of RBP7 mRNA Expression in Breast Cancer Tissues with Tumor Immune Cell Infiltration and Prognosis Based on TCGA Database
10.3969/j.issn.1671-7414.2024.02.014
- VernacularTitle:基于TCGA数据库分析乳腺癌组织RBP7 mRNA表达与肿瘤免疫细胞浸润及预后的相关性
- Author:
Ran CHEN
1
;
Weiyi WANG
;
Yining YANG
Author Information
1. 江苏省肿瘤医院,江苏省肿瘤防治研究所,南京医科大学附属肿瘤医院检验科,南京 210009
- Keywords:
breast cancer;
the cancer genome atlas(TCGA);
retinol binding protein 7(RBP7);
tumor-infiltrating immune cells(TIICs)
- From:
Journal of Modern Laboratory Medicine
2024;39(2):75-80,180
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of retinol binding protein 7(RBP7)in breast cancer by bioinformatics.Methods R sofrware was used to explore the differential expression of the RBP7 gene in breast cancer by the cancer genome atlas(TCGA)dataset and the human protein atlas(HPA).Relationship between RBP7 and clinical data of breast cancer was evaluated by Kaplan-Meier survival analysis and receiver operating characteristic(ROC)curves.Correlation between high and low RBP7 expression groups and different tumor-infiltrating immune cells(TIICs)were analyzed based on the TCGA database.Gene set enrichment analysis(GSEA)was used to assess the distribute trends of RBP7 in gene tables sorted by phenotypic relatedness.Results RBP7 mRNA expression levels were down-regulated in breast cancer compared to paracancerous tissues,which were expressed in the nucleus.ROC curve analysis showed that the area under curve(AUC)of RBP7 for the diagnosis of breast cancer was 0.943(95%CI:0.926～0.960),and the best cut-off value of RBP7 was 6.29,with a sensitivity and specificity of 82.32%and 93.69%,respectively.Kaplan-Meier survival analysis showed that low expression of RBP7 was associated with overall survival rate in breast cancer patients(HR=0.68,95%CI:0.49～0.93,P=0.017),indicating that RBP7 was an independent risk factor for breast cancer.Spearman correlation showed that RBP7 was positively associated with pDC cells and NK cells(r=0.290,0.253,all P<0.05),and negatively associated with Th2 cells(r=-0.217,P<0.05)in breast cancer.GSEA showed that RBP7 was enriched in pathways such as adipogenesis,ribosome,peptiden ligand binding receptors,and calcium signaling pathway(all P<0.001).Conclusion RBP7 affects the occurrence and development of breast cancer,which may be a potential biomarker and therapeutic target for breast cancer.
