Shuxuening injection protects rats from cerebral ischemia through the Wnt/β-catenin signaling pathway
10.16557/j.cnki.1000-7547.2024.01.013
- VernacularTitle:舒血宁注射液通过Wnt/β-catenin信号通路保护大鼠缺血性脑损伤
- Author:
Ming XIE
1
;
Zaogen CHEN
;
Weihua JU
Author Information
1. 南昌大学抚州医学院人体解剖学教研室,抚州 344000
- Keywords:
Wnt/β-catenin signaling pathway;
Shuxuening injection(SXN);
cerebral ischemia;
hippocampus;
rat
- From:
Chinese Journal of Neuroanatomy
2024;40(1):91-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Shuxuening injection(SXN)on Wnt/β-catenin signaling pathway in hippocampus of rats with cerebral ischemia.Methods:SD rats were divided into three groups:Sham operation group(Sham),middle cerebral artery occlusion group(MCAO)and MCAO+SXN treatment group(MCAO+SXN).The model of cerebral ischemia in rats was prepared by MCAO.The rats with cerebral ischemia were treated with SXN by caudal vein injection.Zea-Longa scoring criteria and balance beam test were employed to evaluated neurological function of rats.HE staining were used to observe the changes of inflammatory cells infiltration the hippocampal CAI region.The expression of β-catenin in hippocampal CA1 region was observed by immunofluorescence staining.The mRNA and pro-tein expressions of caspase-3,cyclooxygenase-2(COX-2)and endothelial nitric oxide synthetase(eNOS)in hippocam-pus were detected by real time RT-PCR and Western Blot,respectively.Results:SXN can SXN can improve the neuro-logical dysfunction of cerebral ischemia rats.The inflammatory cells infiltration in hippocampal CAI region was decreased,and the expression of β-catenin,caspase-3 and COX-2 was decreased,while the expression of eNOS was upregulated.Conclusion:SXN protects against cerebral ischemia by inhibiting Wnt/β-catenin signaling pathway against inflammation response,oxidative stress and apoptosis of nerve cells in rats.
