HNRNPF promotes the proliferation,migration and invasion of prostate cancer
10.3969/j.issn.1672-8467.2024.01.004
- VernacularTitle:HNRNPF促进前列腺癌的增殖、迁移和侵袭
- Author:
Shi-Yu WANG
1
;
Xue-Feng XIE
;
Gang CHEN
Author Information
1. 复旦大学附属金山医院临床医学研究中心, 上海 201508
- Keywords:
prostate cancer;
heterogeneous nuclear ribonucleoproteins F(HNRNPF);
cell proliferation;
cell migration;
cell invasion
- From:
Fudan University Journal of Medical Sciences
2024;51(1):25-33,68
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and clinical relevance of heterogeneous nuclear ribonucleoproteins F(HNRNPF)in prostate cancer and its effect on the proliferation,migration and invasion of prostate cancer cells.Methods The expression and immune infiltration characteristics of HNRNPF in prostate cancer and its correlation with the clinicopathological characteristics of prostate cancer patients were analyzed using TCGA database and GEO database.The HNRNPF gene was silenced by RNA interference in prostate cancer cell PC-3 and DU145,then the changes in cell proliferation ability was detected by CCK-8,EdU and colony formation assays,and the changes in cell migration and invasion abilities were detected by Transwell and wound-healing assays.Results The expression of HNRNPF was significantly increased in prostate cancer compared with normal prostate tissue and significantly associated with T stage,Gleason score,prostate specific antigen and the infiltration level of multiple immune cells of prostate cancer patients.The prostate cancer patients with high expression of HNRNPF had shorter overall survival and disease-specific survival.HNRNPF silencing decreased the proliferation,migration,and invasion abilities of prostate cancer cells PC-3 and DU145.Conclusion HNRNPF is a gene that is highly expressed in prostate cancer,has significant clinical relevance,and can promote the proliferation,migration,and invasion of prostate cancer cells.
