Influences of long non-coding RNA ABHD11-AS1 on the invasion and migration of gastric cancer cells by regulating miR-542-3p/MAP3K11
10.3760/cma.j.cn.115807-20220401-00081
- VernacularTitle:长链非编码RNA ABHD11-AS1通过调节miR-542-3p/MAP3K11对胃癌细胞侵袭和迁移的影响
- Author:
Xianhao YING
1
;
Jipeng LI
Author Information
1. 宁波大学附属人民医院消化内科，宁波 315000
- Keywords:
Long non-coding RNA ABHD11-AS1;
miR-542-3p;
MAP3K11;
Gastric cancer;
Migration;
Invasion
- From:
Chinese Journal of Endocrine Surgery
2023;17(5):619-623
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of long non-coding RNA ABHD11-antisense RNA1 (LncRNA ABHD11-AS1) on the invasion and migration of gastric cancer (GC) cells by regulating miR-542-3p/MAP3K11.Methods:After routine culture, gastric cancer cells BGC-823 were divided into si-NC group, si-LncRNA ABHD11-AS1 group, si-LncRNA ABHD11-AS1+miR-NC inhibitor group, si-LncRNA ABHD11-AS1+miR-542-3p inhibitor, si-LncRNA ABHD11-AS1+miR-542-3p inhibitor+si-con group and ABHD11-AS1+miR-542-3p inhibitor+si-MAP3K11 group. The interaction relationship between LncRNA ABHD11-AS1, miR-542-3p, and MAP3K11 was predicted and verified. The levels of LncRNA ABHD11-AS1, miR-542-3p, and MAP3K11 mRNA in GC cells were detected by qRT-PCR, and the migration and invasion ability of GC cells were detected by the Transwell method, and the protein expression levels of MAP3K11, MMP-2, and MMP-9 in GC cells were detected by Western blot.Results:The targeting relationship between LncRNA ABHD11-AS1 and miR-542-3p, miR-542-3p and MAP3K11 were verified ( P<0.05). Compared with the si-NC group [ (184.09±17.32) and (87.64±7.54) ], the migration and invasion capacity of gastric cancer cells in the si-LncRNA ABHD11-AS1 group were significantly reduced [ (68.35±6.21) and (28.23±3.05) ( P all<0.05) ]; Compared with the si-LncRNA ABHD11-AS1+miR-NC inhibitor group [ (75.39±7.08) and (31.26±3.15) ], the migration and invasion capacity of gastric cancer cells in the si-LncRNA ABHD11-AS1+miR-542-3p inhibitor group were significantly increased [ (138.24±12.58) and (61.23±5.86) ( P all<0.05) ]; Compared with the si-LncRNA ABHD11-AS1+miR-542-3p inhibitor+si-con group [ (145.33±13.09) and (65.45±6.08) ], the migration and invasion ability of gastric cancer cells in the si-LncRNA ABHD11-AS1+miR-542-3p inhibitor+si-MAP3K11 group were significantly reduced [ (108.36±9.87) and (145.33±13.09) ( P all<0.05) ]. Conclusion:Down-regulating of LncRNA ABHD11-AS1 could suppress the migration and invasion of GC cells by miR-542-3p/MAP3K11 axis.
