Relationship between the expression of PNCK,EGFR and EGFR gene mutation in non-small cell lung cancer
10.13315/j.cnki.cjcep.2023.11.012
- VernacularTitle:非小细胞肺癌中PNCK、EGFR表达与EGFR基因突变状态的相关性
- Author:
Jia LUO
1
;
Chenggang YANG
Author Information
1. 云南省肿瘤医院病理科,昆明 650100
- Keywords:
lung neoplasm;
non-small cell lung cancer;
PNCK;
EGFR;
immunohistochemitry
- From:
Chinese Journal of Clinical and Experimental Pathology
2023;39(11):1345-1350
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the correlation of PNCK and EGFR expression with EGFR gene mutation status in non-small cell lung cancer(NSCLC).Methods Immunohisto-chemical staining was used to detect the expression of PNCK and EGFR in 256 cases of invasive lung carcinoma,42 cases of inva-sive precancerous lesions and 40 cases of paracancerous lung tis-sues,EGFR gene amplification and mutation were detected by next-generation sequencing.The relationship between the ex-pression of PNCK and EGFR protein and the clinicopathological features of NSCLC,the mutation status of EGFR gene and the correlation between them were analyzed.GEPIA database was used to analyze the correlation between PNCK and EGFR gene expression in lung adenocarcinoma and lung squamous cell carci-noma,and Kaplan-Meier Plotter database was used to analyze the effect of PNCK on the prognosis of lung cancer.Results Expression of PNCK and EGFR in lung invasive carcinoma(53.5％,137/256;47.7％,122/256)was higher than those of pre-invasive lesions(47.6％,20/42;31.0％,13/42)and paracancer tissue(22.5％,9/40;7.5％,3/40),pair-to-pair comparison among the three groups had statistical significance(P＜0.05).The high expression of PNCK protein was correla-ted with the degree of cancer differentiation,TNM stage,lymph node metastasis and distant metastasis(P＜0.05).The high expression of EGFR protein was correlated with TNM stage,lymph node metastasis and distant metastasis(P＜0.05).There was a negative correlation between PNCK and EGFR pro-tein expression in NSCLC tissues(r=-0.208,P＜0.05).The expression of EGFR protein in NSCLC with EGFR gene mu-tation and amplification was higher than that of wild type(P＜0.05).There was no correlation between PNCK protein expres-sion and EGFR gene mutation and amplification(P＞0.05).Bioinformatics database showed that PNCK and EGFR gene ex-pression were positively correlated in lung adenocarcinoma and lung squamous cell carcinoma(r=0.15,P＜0.05;r=0.30,P＜0.05),PNCK high expression group had a shorter overall survival rate in lung cancer(HR:1.28,95％CI:1.09-1.51,P=0.003 1).Conclusion The high expression of PNCK is associated with the occurrence,development,and poor prognosis of NSCLC,and negatively correlated with EGFR pro-tein expression.It is expected to become a potential therapeutic target for EGFR mutations in NSCLC.
