Application of ALK antibody clone 1A4 in the diagnosis of non-small cell lung cancer with ALK fusion
10.13315/j.cnki.cjcep.2023.11.005
- VernacularTitle:ALK(1A4)在ALK融合非小细胞肺癌诊断中的应用
- Author:
Yue WANG
1
;
Yan JIN
;
Qiang ZHENG
;
Qianqian XUE
;
Wenhua JIANG
;
Xu CAI
;
Yuan LI
Author Information
1. 复旦大学附属肿瘤医院病理科,上海 200032;复旦大学上海医学院肿瘤学系,上海 200032
- Keywords:
lung neoplasm;
non-small cell lung cancer;
ALK;
ALK(1A4)antibody;
ALK(D5 F3)antibody;
immunohisto-chemistry
- From:
Chinese Journal of Clinical and Experimental Pathology
2023;39(11):1305-1310
- CountryChina
- Language:Chinese
-
Abstract:
Purpose The aim of this study is to explore the application value of ALK(1A4)in ALK fused non-small cell lung cancer(NSCLC).Methods A total of 1 035 NSCLC specimens were collected.The expression of ALK(1A4)and ALK(D5F3)antibodies was detected by immunohistochemical(IHC)staining,and their consistency was analyzed.FISH and next-generation sequencing(NGS)were used to detect ALK positive,and the sensitivity and specificity of ALK in the two groups were evaluated.Results ALK fused NSCLC patients accounted for about 5.4％(56/1 035).The positive rate of ALK(1A4)was 7.2％(75/1 035),and that of ALK(D5F3)was 5.7％(59/1 035).The consistency between them was high,with a Kappa value of 0.874.The consistency of ALK(1A4)and ALK(D5 F3)antibodies with FISH was high,with Kappa values of 0.845 and 0.954,respectively.The consisten-cy of ALK(1A4)and ALK(D5F3)with NGS was also high,with Kappa values of 0.836 and 0.988,respectively.According to the FISH results,the sensitivities of ALK(1A4)and ALK(D5F3)antibodies were 100％and 98.2％,and the specifici-ties were 98.1％and 99.6％,respectively.Conclusion ALK(1A4)antibody has high sensitivity and slightly low specificity,and can be used for clinical screening of ALK fused NSCLC.
