Impacts of butorphanol on the proliferation,apoptosis and migration of esophageal cancer cells by regulating CCL2-CCR2 axis
10.3969/j.issn.1005-6483.2024.02.013
- VernacularTitle:布托啡诺调节CCL2-CCR2轴对食管癌细胞增殖、凋亡和迁移的影响
- Author:
Fan JIANG
1
;
Keqi XIE
Author Information
1. 621000 四川绵阳,电子科技大学医学院附属绵阳医院/绵阳市中心医院麻醉科
- Keywords:
butorphanol;
esophageal cancer cells;
CC chemokine ligand 2-CC chemokine receptor 2 axis;
proliferation;
apoptosis;
migration;
invasion
- From:
Journal of Clinical Surgery
2024;32(2):168-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impacts of butorphanol on the proliferation,apoptosis and migration of esophageal cancer cells and its regulation on CCL2-CCR2 axis.Methods Human esophageal cancer KYSE30 cells were treated with 0～400 ng/ml of butorphanol gradient concentration,and the cell proliferation was detected by MTT method;KYSE30 cells were grouped into control group,butorphanol L group,butorphanol M group,butorphanol H group and butorphanol H+CCL2 group,EdU method was applied to detect cell proliferation;cell apoptosis was detected by Hoechst method;cell migration was detected by cell scratch test;Transwell method was applied to detect cell invasion;Western blot was applied to verify the expression of CC chemokine ligand 2(CCL2)and CC chemokine receptor 2(CCR2).Results Butorphanol at the concentrations of 50 ng/ml,100 ng/ml,200 ng/ml and 400 ng/ml obviously inhibited the proliferation of KYSE30 cells;compared with the control group,the proliferation,migration and invasion of KYSE30 cells and the expression of CCL2 and CCR2 proteins in butorphanol L,M,H groups and butorphanol H+CCL2 groups were obviously decreased(P＜0.05);compared with butorphanol H group,the cell proliferation,migration,invasion abilities and the expression levels of CCL2 and CCR2 proteins in butorphanol H+CCL2 group were obviously increased(P＜0.05).Conclusion Butorphanol can obviously inhibit the proliferation,migration and invasion of esophageal cancer KYSE30 cells and promote cell apoptosis,which may be related to its inhibition of CCL2-CCR2 axis.
