Positive effects of neuroligin-1,-2 on oligodendrocyte differentiation and myelination
10.16098/j.issn.0529-1356.2024.02.001
- VernacularTitle:神经连接蛋白1、2对少突胶质细胞分化与髓鞘形成的促进作用
- Author:
Xiao-Jiao ZHANG
1
;
Ming-Mei ZHU
;
Jia-Yan WANG
;
Xin TANG
Author Information
1. 南通大学神经再生重点实验室,神经再生协同创新中心,江苏 南通 226001
- Keywords:
Neuroligin;
Oligodendrocyte;
Myelination;
Immunofluorescence;
Rat
- From:
Acta Anatomica Sinica
2024;55(2):125-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of neuroligin-1,-2(NLGN-1,-2)on oligodendrocyte(OLs)differentiation and myelination in the central nervous system.Methods OLs were cultured in vitro in the presence of different concentrations of NLGN-1 and NLGN-2.Morphological differentiation of OLs was observed by immunofluorescent staining and mRNA expression levels of myelin-associated genes were detected by Real-time PCR.Western blotting was used to detect the expression of myelin-related proteins.Results NLGN-1,-2 accelerated the differentiation of oligodendrocyte precursor cells(OPCs)into mature OLs,and promoted the ability of myelin sheath formation.In vitro culture conditions,the dosage of 500 μg/L had the best promotion effect on OLs differentiation and maturation,and NLGN-2 had better promoting effect than that of NLGN-1.Furthermore,the mRNA expression levels of myelin-associated genes myelin protein P0(MPZ),myelin basic protein(MBP)increased after the neuroligins treatments detected by Real-time PCR.Western blotting result showed that the expressions of MBP and MPZ increased significantly after 500 μg/L treatment with NLGN-1 and NLGN-2 for 12 hours.Conclusion NLGN-1,-2 promote OLs differentiation and myelination.The positive effect of NLGN-2 is greater than that of NLGN-1 significantly,suggesting that the treatment with inhibitory synaptic-associated cytokines may improve the ability of myelin sheath formation in the central nervous system.
