Serum Metabolomics Characteristics of Chronic Atrophic Gastritis Patients with Liver-Stomach Qi Stagnation Syndrome and Spleen-Stomach Weakness Syndrome
10.13359/j.cnki.gzxbtcm.2024.01.002
- VernacularTitle:慢性萎缩性胃炎肝胃气滞证和脾胃虚弱证患者血清代谢组学特征研究
- Author:
Yu-Yi CHEN
1
;
Juan-Juan LI
;
Hong-Liang WANG
;
Shao-Ju GUO
;
Bin HUANG
Author Information
1. 广州中医药大学第四临床医学院,广东深圳 518000;深圳市中医院,广东深圳 518000
- Keywords:
chronic atrophic gastritis(CAG);
traditional Chinese medicine(TCM)syndrome types;
liver-stomach qi stagnation syndrome;
spleen-stomach weakness syndrome;
metabolomics;
biomarkers
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2024;41(1):7-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the metabolomics characteristics of chronic atrophic gastritis(CAG)patients with liver-stomach qi stagnation and spleen-stomach weakness syndromes based on non-targeted metabolomics technology,and to identify the serum differentiated metabolites related to traditional Chinese medicine(TCM)syndrome of CAG patients,so as to provide a reference for the objectification of syndrome differentiation.Methods Sixty patients with CAG were included,including 30 cases of liver-stomach qi stagnation syndrome and 30 cases of spleen-stomach weakness syndrome.Fasting blood of 5 mL was collected from the cubital vein of patients in the two groups,and the serum levels of metabolites were detected by ultra-high-performance liquid chromatography-mass spectrometry(UPLC-MS)methods.The principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA),and cluster analysis were used to screen the differentiated metabolites of CAG patients with liver-stomach qi stagnation syndrome and spleen-stomach weakness syndrome.Finally,metabolite pathway analysis was performed for the obtained differentiated metabolites using the KEGG database.Results The results for the screening of differentiated metabolites showed that significant differences of amino acid derivatives and small peptide metabolites were presented between CAG patients with liver-stomach qi stagnation syndrome and CAG patients with spleen-stomach weakness syndrome.The amino acid derivatives consisted of N-acetylglycine,histamine,O-phosphoserine,selenomethylselenocysteine,and methyl-tyrosine.And the small peptide metabolites consisted of tyrosine-leucine-phenylalanine,histidine-alanine-glutamate-lysine,L-asparagine-L-proline-L-serine,and L-isoleucine-L-isoleucine.Conclusion Differences in amino acid metabolism exist between CAG patients with liver-stomach qi stagnation syndrome and those with spleen-stomach weakness syndrome,and metabolites such as N-acetylglycine,intermethyltyrosine,and O-phosphoserine may be the potential biomarkers for distinguishing liver-stomach qi stagnation syndrome from spleen-stomach weakness syndrome in CAG patients.
