Effects of respiratory syncytial virus infection on nasal epithelial barrier function in chronic rhinosinusitis with polyps
10.16066/j.1672-7002.2024.01.007
- VernacularTitle:呼吸道合胞病毒感染对慢性鼻窦炎伴鼻息肉上皮屏障功能的影响
- Author:
Ziqing KANG
1
;
Jingxing YANG
;
Xiangdong WANG
;
Chao CAI
Author Information
1. 首都医科大学附属北京佑安医院耳鼻咽喉头颈外科,北京 100069
- Keywords:
Sinusitis;
Nasal Polyps;
Respiratory Syncytial Viruses;
Epithelial Cells;
Nasal Mucosa;
Tight Junctions;
human nasal epithelial cells
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2024;31(1):31-35
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the effect of respiratory syncytial virus(RSV)infection on the expression of key factors in the epithelial barrier of the human nasal epithelial cells(hNECs)derived from patients with chronic rhinosinusitis with nasal polyps(CRSwNP)and normal control mucosa.METHODS RSV with different multiplicity of infection(MOI)(0.1 and 0.3)infected hNECs derived from patients with CRSwNP(n=21)and normal control mucosa(n=9)for 24 h and 48 h,respectively.To detect the gene expression ZO-1,ZO-2,Claudin-1,Claudin-4,Occludin,E-cadherin and N-cadherin,total RNA was extracted and reverse transcribed into cDNA for real-time fluorescence quantification PCR.RESULTS The relative expression level of ZO-1,ZO-2,Claudin-1,Claudin-4,Occludin,E-cadherin and N-cadherin were decreased in hNECs post RSV infection.However,there was a statistical difference only in hNECs derived from CRSwNP(P<0.05).There was no significant difference in hNECs infected with RSV between eosinophilic CRSwNP(ECRSwNP)and non-eosinophilic CRSwNP(nonECRSwNP).CONCLUSION RSV infection could disrupt the epithelial barrier of the nasal mucosa,and patients with CRSwNP are more severely affected by RSV infection compared to healthy controls.The impact of RSV infection on mucosa between ECRSwNP group and nonECRSwNP group was no significant difference.
