Fabrication of a Scaffold for Bone Regeneration and Differentiation of Mesenchymal Stem Cells.
- Author:
Ki Chan AN
1
;
Jung Wook SHIN
;
Yoon Jun KIM
;
Dae Won JUNG
;
Dae Hyun PARK
;
Dong Min KIM
Author Information
1. Department of Orthopedic Surgery, College of Medicine, University of Inje, Busan Paik Hospital, Busan, Korea. 97111033@hanmail.net
- Publication Type:Original Article
- Keywords:
Mesenchymal stem cells;
Intermittent hydrostatic pressure;
Microenvironment;
Physical stimuli;
Osteogenesis;
Bone regeneration
- MeSH:
Animals;
Bone Regeneration;
Bone Substitutes;
Collagen Type I;
Durapatite;
Hydrostatic Pressure;
Mesenchymal Stromal Cells;
Osteogenesis;
Porosity;
Rabbits;
Seeds
- From:Journal of Korean Orthopaedic Research Society
2012;15(1):1-10
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This in-vitro study aimed to confirm the clinical potential of a newly suggested bone substitute fabricated by a bio-plotting system. MATERIALS AND METHODS: A 3-dimensional, PCL (polycaprolactone)-based bone substitute was fabricated by a bio-plotting system, which enables us to control the shape and porosity. Mesenchymal stem cells(MSCs) from a New Zealand white rabbits were seeded to the bone substitute. Through 2-week experiment, morphological and various biological responses were assessed depending on the use of osteogenic media and/or mechanical stimuli. RESULTS: The viability of cells was confirmed. Other findings in relation to proliferation and differentiation were also confirmed to be biocompatible. Specifically, the activity of ALP was increased in the groups using osteogenic media along the time. And the expressions collagen type I was more affected by intermittent hydrostatic pressure rather than by osteogenic media. But those of Cbfa-1 was affected by osteogenic media and mechanical stimuli, as well. CONCLUSION: The potential of a newly fabricated and suggested material was confirmed. However, further long term and animal study are recommanded for the clinical application.
