Construction and evaluation of novel self-assembled nanoparticles of Herpetospermum caudigerum Wall.
10.16438/j.0513-4870.2023-0731
- VernacularTitle:波棱瓜子新型自组装纳米粒的构建及评价
- Author:
Yu-wen ZHU
1
;
Xiang DENG
2
;
Li CHEN
2
;
Jian-tao NING
2
;
Yu-ye XUE
2
;
Bao-de SHEN
3
;
Ling-yu HANG
2
;
Hai-long YUAN
1
Author Information
1. Department of Pharmacy, Air Force Medical Center, PLA, Air Force Medical University, Beijing 100142, China; Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, China
2. Department of Pharmacy, Air Force Medical Center, PLA, Air Force Medical University, Beijing 100142, China
3. Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, China
- Publication Type:Research Article
- Keywords:
italic>Herpetospermum caudigerum Wall.;
micro-precipitation method;
self-assembled;
ecoction;
nanoparticle
- From:
Acta Pharmaceutica Sinica
2024;59(2):448-454
- CountryChina
- Language:Chinese
-
Abstract:
It has become an industry consensus that self-assembled nanoparticles (SAN) are formed by molecular recognition of chemical components in traditional Chinese medicine during the decoction process. The insoluble components in the decoction are mostly in the form of nanoparticles, which can improve the problem of poor water solubility. However, the transfer rate of these insoluble components in the decoction is still very low, which limits the efficacy of the drug. This study aimed to refine the traditional decoction self-assembly phenomenon. The self-assembled nanoparticles were constructed by micro-precipitation method (MP-SAN), and characterized by particle size, zeta potential, stability index and morphology. The formation of MP-SAN and alterations in related physicochemical properties were evaluated using modern spectroscopic and thermal analysis techniques. The quality value transmitting pattern of lignan components within the MP-SAN was assessed via high performance liquid chromatography (HPLC). The MP-SAN showed sphere-like structure with uniform morphology, particle size of (245.3 ± 3.2) nm, polydispersity index (PDI) of (0.13 ± 0.03), zeta potential of (-48.9 ± 5.9) mV and stability index (SI) of (86.05% ± 2.27%). Comprehensive analyses using ultraviolet visible spectroscopy, Fourier transform infrared spectroscopy, differential scanning calorimetry, and other techniques confirmed molecular recognition between the decoction and ethanol extraction, leading to electron rearrangement under the influence of non-covalent bonding. This resulted in the formation of nanoparticles possessing superior thermal stability. As determined by HPLC, the encapsulation rates of the index components in the MP-SAN were all greater than 75% (dehydrodiconiferyl alcohol: 77.00%; herpetolide A: 78.57%; herpetrione: 94.53%), and the transfer rates were all higher than 65% (dehydrodiconiferyl alcohol: 96.01%; herpetolide A: 67.86%; herpetrione: 65.55%), which were 1.34, 1.38 and 4.81 times compared with those of the traditional decoction. In summary, this study successfully constructed the MP-SAN based on micro-precipitation method to achieve high transfer rate and high encapsulation rate of insoluble components in docoction, which provides a pharmaceutics idea for the efficient utilization of pharmacodynamic substance basis of traditional Chinese medicine.
