Identification, expression and protein interaction analysis of Aux/IAA and ARF gene family in Senna tora L.
10.16438/j.0513-4870.2023-0666
- VernacularTitle:决明Aux/IAA和ARF基因家族鉴定、表达及蛋白互作分析
- Author:
Zhao FENG
1
;
Shi-peng LIU
1
;
Rui-hua LÜ
1
;
Rui-hua LÜ
1
;
Xiao-chen HU
2
;
Ming-ying ZHANG
2
;
Ren-jun MAO
3
;
Gang ZHANG
2
Author Information
1. College of Medical Technology, Shaanxi University of Chinese Medicine, Xianyang 712046, China
2. Key Laboratory for Research of "Qin Medicine" of Shaanxi Administration of Chinese Medicine, College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China
3. College of Life Science, Yan'an University, Yan'an 716000, China
- Publication Type:Research Article
- Keywords:
italic>Senna tora;
italic>Aux/IAA;
auxin response factor;
hormone;
protein interaction
- From:
Acta Pharmaceutica Sinica
2024;59(3):751-763
- CountryChina
- Language:Chinese
-
Abstract:
The early response of plant auxin gene family Aux/IAA (auxin/indole-3-acetic acid) and its interaction with auxin response factor (ARF) are important pattern to regulate plant growth and development. This work identified 28 StoIAA and 24 StoARF members based on the whole genome data of the medicinal plant Senna tora L., which were classified into 10 and 8 subfamilies, respectively. Phylogenetic tree and collinearity analysis showed that S. tora has close evolutionary relationship with the IAA and ARF homologous genes of Glycine max, Medicago truncatula, and the segment duplication events dominate the expansion of StoIAA and StoARF. Gene structure analysis showed that the vast majority of StoIAA and StoARF contain characteristic conserved domain. Transcriptome data showed that StoIAAs and StoARFs were expressed in leaves, roots and seeds, some members had tissue specific expression. The StoIAA and StoARF promoter region most contain functional elements related to stress response, growth and development, hormone induction and secondary metabolism. In addition, gene expression analysis showed that many StoIAAs and StoARFs can quickly respond to drought and salt stress and exhibited same expression patterns under both stress condition. The yeast two-hybrid experiment confirmed that StoARF8 and StoARF10 exhibit varying degrees of interaction with multiple StoIAA proteins, respectively. The above results provide a basis for further biological functional analysis of the Aux/IAA and ARF gene family of S. tora.
