The Changes of Pro- and Anti-inflammatory Cytokines in Both Serum and Gastrocnemius Muscle of Mice after 2-hour Postischemic Reperfusion Injury.
10.11637/kjpa.2014.27.2.101
- Author:
Shin Young KIM
1
Author Information
1. Department of Anatomy and Cell Biology, Hanyang University, Korea. 72shin02@hanmail.net
- Publication Type:Original Article
- Keywords:
Ischemia;
Reperfusion;
Pro-inflammatory cytokine;
Anti-inflammatory cytokine
- MeSH:
Anesthesia;
Animals;
Chemokine CCL3;
Chemokine CCL5;
Cytokines*;
Dislocations;
Enzyme-Linked Immunosorbent Assay;
Heart Atria;
Humans;
Iliac Artery;
Interleukin-2;
Interleukin-3;
Interleukin-5;
Interleukin-6;
Ischemia;
Male;
Mice*;
Muscle, Skeletal*;
Muscles;
Reperfusion;
Reperfusion Injury*;
Serum
- From:Korean Journal of Physical Anthropology
2014;27(2):101-111
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study aimed to investigate the inflammatory changes and their main indicators according to the time-period of postischemic reperfusion injury confirmed by analyzing changes of both pro-inflammatory and anti-inflammatory cytokines in the skeletal muscle and serum. By using 12-week-old male ICR strain mice were grouped into sham control and 8 different time-periods of reperfusion groups (0, 0.5, 1, 2, 4, 8, 16, 24 hours). Left common iliac artery of each mice in the reperfusion group was devascularized by a vascular clamp for 2 hours. Once anesthesia was applied to the experimental animals, blood serum was obtained from right heart atrium on the difference time-period of reperfusion (0-, 0.5-, 1-, 2-, 4-, 8-hour, respectively). Then, tissue fluid was collected in calf muscles (gastrocnemius muscle) after the mice were sacrificed by cervical dislocation. By using these serum and tissue fluids, enzyme-linked immunosorbent assay (ELISA) was used to analyze both pro-inflammatory cytokines (Eotaxin, IFNgamma, IL-1alpha, IL-1beta, IL-2, IL-3, IL-5, IL-6, MCP-1, MDC, MIP-1alpha, RANTES, TARC, TCA-3) and anti-inflammatory cytokines (IL-4, IL-10). Consequently, there were significant differences of pro-inflammatory cytokines levels in the skeletal muscle of 0-hour reperfusion group (p<.05) and those in the serum of 0-, 1-, 2-, 4-, 8-, 16-hour reperfusion groups (p<.05). In the serum of 4-hour reperfusion group, the presence of anti-iflammatory cytokines was significant from other groups (p<.05). By the comparison with the control group, furthermore, pro-inflammatory cytokines in the serum of 2-, 4-, 16-hour reperfusion group and anti-inflammatory cytokines in the serum of 4-hour reperfusion group were considerably different (p<.05). To sum up, changes of cytokine levels according to the time-period of reperfusion were considerably different in the serum rather than the tissue fluids from the skeletal muscle. In particular, IL-6 and MCP-1 in the serum showed higher density in 4- and 16-hour reperfusion groups so that they could be considered as the main indicator of pro-inflammatory cytokines.
