MicroRNA-100-5p Regulates Melanogenesis in Alpaca Melanocytes by Targeting FGF21
10.13865/j.cnki.cjbmb.2020.12.1472
- Author:
Qiong JIA
1
;
Rui-Wei WANG
1
;
Shi-Xiong HU
1
;
Rui-Wen FAN
1
Author Information
1. Alpaca Bioengineering Laboratory, College of Veterinary Medicine, Shanxi Agricultural University
- Publication Type:Journal Article
- Keywords:
extracellular regulated MAP kinase (ERK);
fibroblast growth factor 21 (FGF21);
melanogenesis;
microphthalmia-associated transcription factor (MITF);
miR-100-5p
- From:
Chinese Journal of Biochemistry and Molecular Biology
2021;37(2):244-250
- CountryChina
- Language:Chinese
-
Abstract:
Although many microRNAs (miRNAs) are known to function as regulators of coat color and melanogenesis, the underlying molecular mechanisms of miR-100-5p governing melanogenesis were not completely known.The goal of this study was to determine the effect of miR-l()()-5p on melanogenesis in alpaca melanocytes.Fibroblast growth factor 21 (FGF21) is a predicted target gene of miR-100-5p and the luciferase reporter assay demonstrated that miR-100-5p regulates FGF21 by binding to its 3' untranslated region (3'UTR).In this study, alpaca melanocytes were transfected with miR-100-5p, inhibitor and negative control plasmid.Results showed that miR-100-5p overexpression significantly decreased mRNA and protein expression of FGF2\.Meanwhile, the ERK signal pathway was inhibited, with subsequent up-regulation of microphthalmia-associated transcription factor (MITF) , tyrosinase (TYR) and tyrosinase-related protein 2 (TYRP2), which increased melanin production.The results suggest that miR-100-5p may regulate melanogenesis by targeting FGF21 via extracellular regulated MAP kinase (ERK) signaling pathway.
