microRNA-203 Inhibits Proliferation and Migration of Human Corneal Epithelial Cells by Targeting SRC

Author: Wei-Wei XU ¹ ; Guang-Ying LUO ¹
Author Information

1. State Key Laboratory of Optometry and Visual Science, Department of Optometry, Wenzhou Medical University
Publication Type:Journal Article
Keywords: corneal epithelial wound healing; migration; miR-203; proliferation
From: Chinese Journal of Biochemistry and Molecular Biology 2021;37(7):900-907
CountryChina
Language:Chinese
Abstract: Corneal epithelial wound healing (CEWH) is vital for maintaining tissue integrity and transparency, but its detailed molecular mechanism is unclear. Previous our study has shown that the expression of miR-203 is dramatically down-regulated during CEWH. Such changes suggest that miR-203 is an important effector of CEWH. In this study, we confirmed down-regulation of miR-203 during CEWH in mice. In vitro experiments, we transfected miR-203 into human corneal epithelial cells (HCECs) to over-express miR-203. The MTS method, EdU detection, flow cytometry, scratch tests and transwell experiments were used to detect changes in cell proliferation, cell cycle, and cell migration after transfection. As a result, it was found that miR-203 can inhibit the proliferation of HCECs (P<0.01). The cell cycle at the G