Proteolysis Pathways via FoxO1 Reduce the Diameter of Myotube under Hypoxia Exposure
10.13865/j.cnki.cjbmb.2021.05.1689
- Author:
Peng-Yu FU
1
;
Peng-Yu FU
2
;
Jie JIA
2
;
Bo-Ya GU
2
;
Li-Jing GONG
2
;
Rong-Xin ZHU
3
;
Bo-Ya GU
4
;
Li-Jing GONG
4
Author Information
1. Department of Physical Education, Northwestern Polytechnical University
2. China Institute of Sport and Health Science, Beijing Sport University
3. Shanghai Research Institute of Sports Science (Shanghai Anti-Doping Center)
4. Key Laboratory of Physical Fitness and Exercise, Ministry of Education, Beijing Sport University
- Publication Type:Journal Article
- Keywords:
autophagy-lysosome pathway (ALP);
forkhead box protein O1 (FoxO1);
hypoxia exposure;
myotube atrophy;
ubiquitin-proteasome pathway (UPP)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2021;37(8):1110-1117
- CountryChina
- Language:Chinese
-
Abstract:
The high altitude/hypoxic environment induced skeletal muscle atrophy is considered to be the interaction of multi-system and multi-organ, but the direct mechanism of hypoxia on muscle cells in this process is not clear. This study intended to investigate the effects of hypoxia exposure on proteins in ubiquitin and autophagy pathways, and explored the possible mechanism of hypoxia induced change of myotube diameter. The expression of myosin, hypoxia inducible factor-1 α (HIF-1α), forkhead box protein O1 (FoxO1), and ubiquitin protease pathway (MuRF1 and Atrogin1) and autophagy lysosomal pathway (p62, Beclin1, LC3) related proteins were detected by Western blot; The integrated optical density (IOD) of Myosin and LC3 was detected by IF. The results showed that the diameters of myotube at 6 h and 12 h were significantly reduced, and the expression of myosin was significantly reduced at 6 h after hypoxia exposure (P<0. 05); the protein levels of HIF-1α and FoxO1 were significantly increased at 6 h (P<0. 05); The expression of MuRF1 in each time points of hypoxia was significantly higher than 0 h (P<0. 05), but no difference of Atrogin1 expression was detected; Compared with 0 h, the expression of p62 was reduced significantly in response to hypoxia. The protein expression of Beclin1 and the IOD of LC3 was increased significantly at 6 h, and the LC3Ⅱ/Ⅰ ratio was significantly higher at 6 h, but significantly lower at 12 h and 24 h (P<0. 05).The results above indicated that the reduction of the myotube diameter of L6 skeletal muscle cells was induced by hypoxia exposure (1% O
