Chemical Composition Analysis of Antibacterial and Anti-inflammatory Functions of Volatile Oils from Pelargonium graveolens L'Herit
10.13865/j.cnki.cjbmb.2021.09.1267
- Author:
Yu-Sheng XU
1
;
Ke SHEN
2
;
Zhi-Lin JIANG
2
;
Heng LIU
3
;
Shi-Yin GUO
4
Author Information
1. Agricultural College, Hunan Agricultural University
2. Institute of Agricultural and Garden Technology, Pu'er University
3. School of Pharmacy and Chemistry, Dali University
4. College of Life Sciences, Hunan University of Science and Technology
- Publication Type:Journal Article
- Keywords:
anti-inflammatory;
antibacterial;
essential oil;
gas chromatography-mass spectrometer (GC-MS);
Pelargonium graveolens L'Herit
- From:
Chinese Journal of Biochemistry and Molecular Biology
2021;37(11):1502-1509
- CountryChina
- Language:Chinese
-
Abstract:
The components of volatile oils are generally complex, and they often have the functions of divergent dissolving surface, insecticidal and antibacterial. However, there are few reports on bacteriostasis, anti-inflammation and antioxidation roles of volatile oils of Pelargonium graveolens L'Herit. The volatile oil of Pelargonium graveolens L’Herit. was extracted by steam distillation, and GC-MS and peak area normalization analysis showed that it mainly contained 30 compounds, and the identified components accounted for 90.26% of the total peak area. The volatile oil of Pelargonium graveolens L'Herit. has a certain inhibitory effect on Candida albicans and Staphylococcus aureus, especially on Candida albicans. The diameter of the bacteriostatic zone is 15.55±1.53 mm by using the oxford cup method. Dexamethasone and low, middle and high doses of volatile oils of Pelargonium graveolens L'Herit. were given after the RAW264. 7 cell inflammatory model and was induced by lipopolysaccharide (LPS = 10.0 μg/mL). ELISA assays showed that it could effectively reduce the expression of IL-1β and TNF-α in inflammatory cells, and the effect of high doses was similar to that of IL-1β and TNF-α in the dexamethasone group. GC-MS was successfully used to determine and identify the chemical constituents of volatile oils from Pelargonium graveolens L'Herit. in this study. We show that the volatile oil of Pelargonium graveolens L'Herit. had certain bacteriostatic activity and effectively reduces the secretion of IL-1β and TNF-α by inflammatory cells. It provides an experimental basis for the development and utilization of volatile oils from Pelargonium graveolens L'Herit.
