Analysis of m

Author: Xiao-Qiong AN ¹ ; Peng XIE ¹ ; Xiao-Xi ZHU ¹ ; Wen-Feng YU ¹ ; Xiao-Qiong AN ² ; Peng XIE ² ; Xiao-Xi ZHU ² ; Wen-Feng YU ² ; Ting-Ting LONG ³
Author Information

1. Key Laboratory of Endemic and Minority Disease, Minority of Education
2. Key Laboratory of Medical Biology, Guizhou Medical University
3. Department of Human Anatomy, School of Basic Medical Science, Guizhou Medical University
Publication Type:Journal Article
Keywords: circular RNA (circRNA); high-throughput sequencing; m; middle cerebral artery occlusion/reperfusion (MCAO/R)
From: Chinese Journal of Biochemistry and Molecular Biology 2022;38(10):1403-1417
CountryChina
Language:Chinese
Abstract: In this study, we performed high-throughput sequencing technology methylated RNA immunoprecipitation sequencing (MeRIP-seq), transcriptome sequencing (RNA-seq) and bioinformatics to analyze the differentially m6A-methylated and differentially expressed profile of circular RNA (circRNA) in middle cerebral artery occlusion/reperfusion (MCAO/R) model, which provided some scientific evidences for revealing the relationship between RNA epigenetic modification and cerebral ischemia reperfusion injury. The neurological deficit scores of mice were evaluated by the Longa score standard. TTC staining was used to detect cerebral infarction volumes, and dot blot was used for the quantification of m