Radiation-induced Hierarchical Topologically Associated Domain Change and Its Role in Cellular Responses to Radiation
10.13865/j.cnki.cjbmb.2022.02.1533
- Author:
Huan TAO
1
;
Xiao-Fei ZHENG
1
;
Xiao-Chen BO
2
;
He-Bing CHEN
2
Author Information
1. Beijing Key Laboratory for Radiobiology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of MilitarySciences
2. Institute of Health Service and Transfusion Medicine, Academy of Military MedicalSciences, Academy of Military Sciences
- Publication Type:Journal Article
- Keywords:
ataxia telangiectasia mutated (ATM);
cellular response toradiation;
DNA damage and repair;
hierarchical structure;
topologically associated domain (TAD)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2022;38(3):298-307
- CountryChina
- Language:Chinese
-
Abstract:
The three-dimensional (3D) genome organization plays an important role in gene regulation. As a basic functional unit of the genome, topologically associated domain (TAD) regulates multiplebiological processes such as gene expression and DNA replication and plays a role in radiation-inducedDNA damage repair. Recent studies showed that TAD is not a completely independent domain butcontains hierarchical internal domains, which could be a new mechanism of gene regulation. To explorethe role of hierarchical TAD in cellular responses to radiation, we apply the OnTAD algorithm, anoptimized nested TAD caller from Hi-C data, to identify hierarchical TAD in 26 Hi-C data from Geneexpression omnibus (GEO) database. These data were from irradiated fibroblasts, lymphoblasts andfibroblasts deficient in the ataxia telangiectasia mutated (ATM) gene with 5 Gy X-ray. We observe thatX-ray can regularly affect the hierarchy of TAD in which high-level TAD is prone to change and low-levelTAD is more conservative. We propose that radiation-induced TAD hierarchy change can regulate cellularresponses to radiation by regulating gene expression, and ATM is a necessary factor for radiation-inducedTAD hierarchy change and recovery. This study provides new insights into the role of the 3D genome inradiation-induced cellular responses from the perspective of hierarchical TAD structures.
